Sandwich-like voltametric immunosensing of interleukin-8 based on β-cyclodextrin/carbon nanotubes and methylthionine chloride@UIO-66 framework.

Autor: Zhou Z; Department of Urology, Meng Chao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People's Republic of China., Lin J; Department of Neonatology, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics and Gynecology and Pediatrics, Fujian Medical University, Fuzhou, People's Republic of China.; Department of Neonatology, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics and Gynecology and Pediatrics, Fujian Medical University, Fuzhou, People's Republic of China.; Fujian Key Laboratory of Women and Children's Critical Diseases Research (Fujian Maternity and Child Health Hospital), Fuzhou, People's Republic of China., Yue D; Ningde City Hospital, Ningde Normal University, Ningde, People's Republic of China., Chen H; Department of Intensive Care Unit, the People's Hospital of Fujian Traditional Medical University, Fuzhou, People's Republic of China. chenhuaiyu2023@126.com., Chen S; Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, Fuzhou, People's Republic of China. dr_chens@163.com.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2024 Jul; Vol. 40 (7), pp. 1357-1363. Date of Electronic Publication: 2024 Apr 25.
DOI: 10.1007/s44211-024-00574-y
Abstrakt: The level of interleukin-8 (IL-8) in the body is an effective factor for the early diagnosis of acute tubular necrosis and oral tumor. In this work, a novel sandwich-like voltametric immunosensor (SVS) of IL-8 was constructed by preparing β-cyclodextrin/carbon nanotube (CD/CNT) to immobilize primary antibody (PAb) of IL-8 and UIO-66-NH 2 MOFs structure to immobilize second antibody (SAb) and methylene blue (Mb) probe. In this designed SVS, the prepared CD/CNT nanohybrid with large surface area and conductivity can immobilize PAb via simple host-guest recognition, and UIO-66-NH 2 provided an ideal platform to accommodate SAb and a large number of Mb molecules as signal-amplifier. In the existence of target IL-8, the current peak of Mb from the SVS assay increases with the increasement of IL-8 level. Through optimizing and adjusting various factors, a wide linearity (0.001-2.5 ng mL -1 ) and low analytical limit (0.2 pg mL -1 ) of IL-8 were realized, so it's expected the developed SVS strategy has significant applications for the detection of IL-8.
(© 2024. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)
Databáze: MEDLINE
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