Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method.

Autor:	Smith P; School of Natural Science, University of Galway, Galway H91 TK33 , Ireland., Le Devendec L; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France., Jouy E; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France., Larvor E; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France., Verner-Jeffreys D; Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK.; UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK., Joseph AW; Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK.; UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK., Stanton E; University of Bristol Veterinary School, Langford, Bristol BS40 5DU, UK., Light E; Veterinary Medicines Directorate (VMD),Woodham Lane, New Haw, Surrey KT15 3LS, UK., Cortinovis L; Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy., Pretto T; Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy., Manfrin A; Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy., Boitard PM; FILI@VET, 15 Rue du Puits, 29600 Saint Martin des Champs, France., Jamin M; FILI@VET, 15 Rue du Puits, 29600 Saint Martin des Champs, France., Keck N; Laboratoire Départemental Vétérinaire de l'Hérault, 34000 Montpellier, France., Le Breton A; Selarl Vet'Eau, 31330 Grenade sur Garonne, France., Thuillier B; Labocéa, Microbiologie vétérinaire, site de Quimper, 29000 Quimper, France., Ravaille C; SOCSA Elevage, 81300 Graulhet, France., Baron S; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France.
Jazyk:	angličtina
Zdroj:	Diseases of aquatic organisms [Dis Aquat Organ] 2024 Apr 25; Vol. 158, pp. 21-25. Date of Electronic Publication: 2024 Apr 25.
DOI:	10.3354/dao03779
Abstrakt:	In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.
Databáze:	MEDLINE
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