Preoperative Vaginal Microbiome as a Predictor of Postoperative Urinary Tract Infection.

Autor:	Occhino JA; Mayo Clinic., Byrnes JN; Mayo Clinic., Wu PY; National Cheng Kung University Hospital, National Cheng Kung University., Walther-Antonio MR; Mayo Clinic., Chen J; Mayo Clinic.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2024 Apr 12. Date of Electronic Publication: 2024 Apr 12.
DOI:	10.21203/rs.3.rs-4069233/v1
Abstrakt:	This is a single Institute, prospective cohort study. We collected twenty- two postmenopausal women with pelvic organ prolapse planning to undergo vaginal hysterectomy with transvaginal pelvic reconstructive surgery, with or without a concomitant anti-incontinence procedure. Vaginal swabs and urine samples were longitudinally collected at five time points: preoperative consult visit (T1), day of surgery prior to surgical scrub (T2), immediately postoperative (T3), day of hospital discharge (T4), and at the postoperative exam visit (T5). Women experiencing urinary tract infection symptoms provided a sample set prior to antibiotic administration (T6). Microbiome analysis on vaginal and urinary specimens at each time point. Region V3-V5 of the 16S ribosomal RNA gene was amplified and sequenced. Sample DNA was analyzed with visit T1, T2, T5 and T6. Six (27.3%) participants developed postoperative urinary tract infection whose vaginal sample at first clinical visit (T1) revealed beta-diversity analysis with significant differences in microbiome structure and composition. Women diagnosed with a postoperative urinary tract infection had a vaginal microbiome characterized by low abundance of Lactobacillus and high prevalence of Prevotella and Gardnerella species. In our cohort, preoperative vaginal swabs can predict who will develop a urinary tract infection following transvaginal surgery for pelvic organ prolapse. Competing Interests: Conflict of interest All authors report no conflicts of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu