C-C motif chemokine receptor 2 and 7 synergistically control inflammatory monocyte recruitment but the infecting virus dictates monocyte function in the brain.

Autor: Winkler CW; Neuroimmunology Section, Laboratory of Neurological Infections and Immunity, Rocky Mountain Laboratories, Department of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA. winklercw@niaid.nih.gov., Evans AB; Neuroimmunology Section, Laboratory of Neurological Infections and Immunity, Rocky Mountain Laboratories, Department of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA.; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Carmody AB; Research Technologies Branch, Rocky Mountain Laboratories, Department of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA., Lack JB; NIAID Collaborative Bioinformatics Resource, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Woods TA; Neuroimmunology Section, Laboratory of Neurological Infections and Immunity, Rocky Mountain Laboratories, Department of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA., Peterson KE; Neuroimmunology Section, Laboratory of Neurological Infections and Immunity, Rocky Mountain Laboratories, Department of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Apr 24; Vol. 7 (1), pp. 494. Date of Electronic Publication: 2024 Apr 24.
DOI: 10.1038/s42003-024-06178-6
Abstrakt: Inflammatory monocytes (iMO) are recruited from the bone marrow to the brain during viral encephalitis. C-C motif chemokine receptor (CCR) 2 deficiency substantially reduces iMO recruitment for most, but not all encephalitic viruses. Here we show CCR7 acts synergistically with CCR2 to control this process. Following Herpes simplex virus type-1 (HSV-1), or La Crosse virus (LACV) infection, we find iMO proportions are reduced by approximately half in either Ccr2 or Ccr7 knockout mice compared to control mice. However, Ccr2/Ccr7 double knockouts eliminate iMO recruitment following infection with either virus, indicating these receptors together control iMO recruitment. We also find that LACV induces a more robust iMO recruitment than HSV-1. However, unlike iMOs in HSV-1 infection, LACV-recruited iMOs do not influence neurological disease development. LACV-induced iMOs have higher expression of proinflammatory and proapoptotic but reduced mitotic, phagocytic and phagolysosomal transcripts compared to HSV-1-induced iMOs. Thus, virus-specific activation of iMOs affects their recruitment, activation, and function.
(© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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