Antimicrobial action and cytotoxicity of hypochlorous acid obtained from an innovative electrolytic device - An in vitro study.

Autor: Souza MA; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Zanella ML; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Vanin GN; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Dallepiane FG; School of Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: dallepianefe@gmail.com., Pizzi CYM; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Ferreira ER; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Fuhr MCS; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Piccolo NM; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., Palhano HS; School of Dentistry, University of Passo Fundo, Passo Fundo, RS, Brazil., da Silva Koch J; School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Souza KR; School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil., da Costa UM; School of Veterinary Medicine, State University of Santa Catarina, Lages, SC, Brazil., Dos Santos VV; School of Medicine, University of Planalto Catarinense, Lages, SC, Brazil., Steier L; School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA; Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India., Bertol CD; School of Pharmacy, University of Passo Fundo, Passo Fundo, RS, Brazil., Figueiredo JAP; School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2024 Jul; Vol. 163, pp. 105966. Date of Electronic Publication: 2024 Apr 08.
DOI: 10.1016/j.archoralbio.2024.105966
Abstrakt: Objective: This study evaluated the antimicrobial effect and cytotoxicity of hypochlorous acid(HClO) obtained from an innovative electrolytic device.
Design: The root canals of fifty extracted human teeth were inoculated with Enterococcus faecalis and divided into 5 groups (n = 10): DW (control); 2% chlorhexidine gel(CHX); 2.5% sodium hypochlorite(NaOCl); 250 ppm HClO and 500 ppm HClO. The counting of colony forming units evaluated the decontamination potential of each group. Cytotoxicity was evaluated after inoculation of tested protocols in fibroblastic cells for 3 min, calculating the cell viability. Specific statistical analysis was performed (α = 5%).
Results: The highest bacterial reduction was observed in experimental groups, with no statistical differences from each other (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences from each other (p < 0.05).
Conclusions: It could be concluded that HClO presented high antimicrobial activity and low cytotoxicity at both tested concentrations.
Competing Interests: Declaration of Competing Interest Dr. Liviu Steier owns IP rights on the Dentaqua device. The rest of authors state that they have no financial affiliation (e.g., employment, direct payment, stock holdings, retainers, consultantships, patent licensing arrangements or honoraria) or involvement with any commercial organization with direct financial interest in the subject or materials discussed in this manuscript, nor have any such arrangements existed in the past three years.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: