Temperature fluctuations during embryo transfer can be mitigated by optimizing transfer protocol.

Autor: Gurner KH; Melbourne IVF, East Melbourne, Victoria, Australia. Electronic address: kathryn.gurner@mivf.com.au., Kelley RL; Melbourne IVF, East Melbourne, Victoria, Australia., Lee LYS; Melbourne IVF, East Melbourne, Victoria, Australia., Gardner DK; Melbourne IVF, East Melbourne, Victoria, Australia.
Jazyk: angličtina
Zdroj: Reproductive biomedicine online [Reprod Biomed Online] 2024 Jun; Vol. 48 (6), pp. 103847. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.rbmo.2024.103847
Abstrakt: Research Question: What impact do variations in embryo transfer catheter loading and movement procedures have on temperature and pH fluctuations during embryo transfer?
Design: Mock embryo transfers were conducted to test the impact of air flow/movement, use of catheter coverings, and the type of workstation used for catheter loading on catheter temperature. A thermocouple probe inserted into the tip of the outer catheter or taped to the exterior of the inner catheter recorded temperature within the catheter every 5 s from time of mock embryo loading (T L ) to 60 s (T L  + 60 s) or from the start of transit (T T ). Fluctuations in culture medium pH in embryo transfer dishes were monitored.
Results: The rate of cooling during transit was faster (all P < 0.05) when catheters were uncovered compared with all covering methods tested. This resulted in a lower catheter temperature at T L  + 20 s (28.43 ± 0.30 °C) compared with catheters covered by plastic tubing (31.4 ± 0.30 °C), paper (31.0 ± 0.26 °C) or paper + thumb (31.1 ± 0.78 °C; all P ≤ 0.05). Temperature was maintained more effectively when catheters were loaded in a crib compared with a heated stage, until initiation of transit, when the rate of temperature decrease was similar. Culture medium pH increased more rapidly when embryo transfer dishes remained on a heated stage during the procedure compared with in an open crib.
Conclusions: Temperature loss during the embryo transfer procedure can be mitigated by reducing the transit time and using catheter coverings. Use of a crib for catheter loading only improved temperature stability while the catheter remained in the crib, not during transit, and reduced pH fluctuations during the procedure.
(Copyright © 2024 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: