Genetic characterization of a captive marmoset (Callithrix jacchus) colony using genotype-by-sequencing.

Autor: Cole SA; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Lyke MM; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Christensen C; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Newman D; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Bagwell A; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Galindo S; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Glenn J; Texas Biomedical Research Institute, San Antonio, Texas, USA., Layne-Colon DG; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Sayers K; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Tardif S; Southwest National Primate Research Center, San Antonio, Texas, USA., Cox LA; Center for Precision Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA., Ross C; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA., Cheeseman IH; Southwest National Primate Research Center, San Antonio, Texas, USA.; Texas Biomedical Research Institute, San Antonio, Texas, USA.
Jazyk: angličtina
Zdroj: American journal of primatology [Am J Primatol] 2024 Jul; Vol. 86 (7), pp. e23630. Date of Electronic Publication: 2024 Apr 24.
DOI: 10.1002/ajp.23630
Abstrakt: The marmoset is a fundamental nonhuman primate model for the study of aging, neurobiology, and many other topics. Genetic management of captive marmoset colonies is complicated by frequent chimerism in the blood and other tissues, a lack of tools to enable cost-effective, genome-wide interrogation of variation, and historic mergers and migrations of animals between colonies. We implemented genotype-by-sequencing (GBS) of hair follicle derived DNA (a minimally chimeric DNA source) of 82 marmosets housed at the Southwest National Primate Research Center (SNPRC). Our primary goals were the genetic characterization of our marmoset population for pedigree verification and colony management and to inform the scientific community of the functional genetic makeup of this valuable resource. We used the GBS data to reconstruct the genetic legacy of recent mergers between colonies, to identify genetically related animals whose relationships were previously unknown due to incomplete pedigree information, and to show that animals in the SNPRC colony appear to exhibit low levels of inbreeding. Of the >99,000 single-nucleotide variants (SNVs) that we characterized, >9800 are located within gene regions known to harbor pathogenic variants of clinical significance in humans. Overall, we show the combination of low-resolution (sparse) genotyping using hair follicle DNA is a powerful strategy for the genetic management of captive marmoset colonies and for identifying potential SNVs for the development of biomedical research models.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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