Photoinactivation Effects of Curcumin, Nano-curcumin, and Erythrosine on Planktonic and Biofilm Cultures of Streptococcus mutans .

Autor:	Ahrari F; Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Nazifi M; Student Research Committee, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran., Mazhari F; Dental Materials Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Ghazvini K; Antimicrobial Resistance Research Center, Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Menbari S; Department of Medical Laboratory Sciences, School of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Fekrazad R; Radiation Sciences Research Center, AJA University of Medical Sciences, Tehran, Iran.; International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research, Network (USERN), Tehran, Iran., Babaei K; Student Research Committee, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran., Banihashemrad A; Department of Restorative, Preventive, and Pediatric Dentistry, University of Bern, Bern, Switzerland.
Jazyk:	angličtina
Zdroj:	Journal of lasers in medical sciences [J Lasers Med Sci] 2024 Apr 16; Vol. 15, pp. e7. Date of Electronic Publication: 2024 Apr 16 (Print Publication: 2024).
DOI:	10.34172/jlms.2024.07
Abstrakt:	Introduction: This in vitro study was conducted to assess the phototoxic effects of curcumin, nano-curcumin, and erythrosine on the viability of Streptococcus mutans (S. mutans) in suspension and biofilm forms. Methods: Various concentrations of curcumin (1.5 g/L, 3 g/L), nano-curcumin (3 g/L), and erythrosine (100 μM/L, 250 μM/L) were examined for their impact on planktonic and biofilm cultures of S. mutans , either individually or in conjunction with light irradiation (photodynamic therapy or PDT). A blue light-emitting diode (LED) with a central wavelength of 450 nm served as the light source. The results were compared to 0.12% chlorhexidine digluconate (CHX) as the positive control, and a solution containing neither a photosensitizer (PS) nor a light source as the negative control group. The dependent variable was the number of viable microorganisms per experiment (CFU/mL). Results: Antimicrobial PDT caused a significant reduction in the viability of S. mutans in both planktonic and biofilm forms, compared to the negative control group ( P <0.05). The highest cell killing was observed in PDT groups with curcumin 3 g/L or erythrosine 250 μmol/L, although the difference with PDT groups using curcumin 1.5 g/L or erythrosine 100 μmol/L was not significant ( P >0.05). Antimicrobial treatments were more effective against planktonic S. mutans than the biofilm form. Conclusion: PDT with either curcumin 1.5 g/L or erythrosine 100 μmol/L may be suggested as an alternative to CHX to inactivate the bacteria in dental plaque or deep cavities. Nano-curcumin, at the selected concentration, exhibited lower efficacy in killing S. mutans compared to Curcumin or erythrosine. Competing Interests: The authors declare that they have no conflict of interest. (Copyright © 2024 J Lasers Med Sci.)
Databáze:	MEDLINE
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