Endophytic fungi of Panax sokpayensis produce bioactive ginsenoside Compound K in flask fermentation.

Autor: Rai S; Institute of Bioresources and Sustainable Development (IBSD), Sikkim Centre, DBT, Tadong, Gangtok, Sikkim, 737102, India.; School of Biotechnology, KIIT-Deemed to be University, Campus XI, Patia, Bhubaneshwar, Odisha, 751024, India., Singh LS; Institute of Bioresources and Sustainable Development (IBSD), Sikkim Centre, DBT, Tadong, Gangtok, Sikkim, 737102, India. sk1laishram@gmail.com.; Department of Microbiology, Assam Down Town University, Guwahati, Assam, 781026, India. sk1laishram@gmail.com., Shaanker RU; School of Ecology and Conservation, Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bellary Road, Bangalore, Karnataka, 560065, India., Jeyaram K; Institute of Bioresources and Sustainable Development (IBSD), Sikkim Centre, DBT, Tadong, Gangtok, Sikkim, 737102, India., Parija T; School of Biotechnology, KIIT-Deemed to be University, Campus XI, Patia, Bhubaneshwar, Odisha, 751024, India., Sahoo D; Institute of Bioresources and Sustainable Development (IBSD), Sikkim Centre, DBT, Tadong, Gangtok, Sikkim, 737102, India.; Department of Botany, University of Delhi, Delhi, 110007, India.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 23; Vol. 14 (1), pp. 9318. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1038/s41598-024-56441-3
Abstrakt: Endophytes of Panax have the potential to produce their host plant secondary metabolites, ginsenosides. Panax sokpayensis, an endemic traditional medicinal plant of the Sikkim Himalayas was explored for the isolation of endophytic fungi. In the present study, we have isolated 35 endophytic fungal cultures from the rhizome of P. sokpayensis and screened for ginsenosides production by HPLC by comparing the peak retention time with that of standard ginsenosides. The HPLC analysis revealed that out of 35 isolates, the mycelial extracts of four fungal endophytes (PSRF52, PSRF53, PSRF49 and PSRF58) exhibited peaks with a similar retention time of the standard ginsenoside, Compound K (CK). LC-ESI-MS/MS analysis led to the confirmation of ginsenoside CK production by the four fungal endophytes which showed a compound with m/z 639.6278, similar to that of standard ginsenoside CK with yield in potato dextrose broth flask fermentation ranging from 0.0019 to 0.0386 mg/g of mycelial mass in dry weight basis. The four prospective fungal endophyte isolates were identified as Thermothielavioides terrestris PSRF52, Aspergillus sp. PSRF49, Rutstroemiaceae sp. strain PSRF53, and Phaeosphaeriaceae sp. strain PSRF58 based on ITS sequencing. The present finding highlights the need for further study on growth optimization and other culture parameters to exploit the endophytes as an alternative source for ginsenoside CK production.
(© 2024. The Author(s).)
Databáze: MEDLINE
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