Resistance training-induced changes in muscle proteolysis and extracellular matrix remodeling biomarkers in the untrained and trained states.

Autor: Scarpelli MC; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil.; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA., Bergamasco JGA; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil.; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA., Godwin JS; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA., Mesquita PHC; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA., Chaves TS; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Silva DG; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Bittencourt D; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Dias NF; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Medalha Junior RA; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Carello Filho PC; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Angleri V; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil., Costa LAR; School of Physical Education and Sport, University of São Paulo - USP, São Paulo, SP, Brazil., Kavazis AN; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA., Ugrinowitsch C; School of Physical Education and Sport, University of São Paulo - USP, São Paulo, SP, Brazil.; Department of Health Sciences and Human Performance, University of Tampa, Tampa, FL, USA., Roberts MD; School of Kinesiology, Nutrabolt Applied and Molecular Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL, 36849, USA. mdr0024@auburn.edu., Libardi CA; MUSCULAB - Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education, Federal University of São Carlos - UFSCar, Rod. Washington Luiz, km 235 - SP 310, São Carlos, SP, 13565-905, Brazil. c.libardi@ufscar.br.
Jazyk: angličtina
Zdroj: European journal of applied physiology [Eur J Appl Physiol] 2024 Sep; Vol. 124 (9), pp. 2749-2762. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1007/s00421-024-05484-5
Abstrakt: Purpose: Resistance training (RT) induces muscle growth at varying rates across RT phases, and evidence suggests that the muscle-molecular responses to training bouts become refined or attenuated in the trained state. This study examined how proteolysis-related biomarkers and extracellular matrix (ECM) remodeling factors respond to a bout of RT in the untrained (UT) and trained (T) state.
Methods: Participants (19 women and 19 men) underwent 10 weeks of RT. Biopsies of vastus lateralis were collected before and after (24 h) the first (UT) and last (T) sessions. Vastus lateralis cross-sectional area (CSA) was assessed before and after the experimental period.
Results: There were increases in muscle and type II fiber CSAs. In both the UT and T states, calpain activity was upregulated and calpain-1/-2 protein expression was downregulated from Pre to 24 h. Calpain-2 was higher in the T state. Proteasome activity and 20S proteasome protein expression were upregulated from Pre to 24 h in both the UT and T. However, proteasome activity levels were lower in the T state. The expression of poly-ubiquitinated proteins was unchanged. MMP activity was downregulated, and MMP-9 protein expression was elevated from Pre to 24 h in UT and T. Although MMP-14 protein expression was acutely unchanged, this marker was lower in T state. TIMP-1 protein levels were reduced Pre to 24 h in UT and T, while TIMP-2 protein levels were unchanged.
Conclusion: Our results are the first to show that RT does not attenuate the acute-induced response of proteolysis and ECM remodeling-related biomarkers.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: