Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov.

Autor: Liunardo JJ; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Messerli S; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Gregotsch AK; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Lang S; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Schlosser K; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Rückert-Reed C; Medical School East Westphalia-Lippe, Bielefeld University, Bielefeld, Germany.; Technology Platform Genomics, Center for Biotechnology, Bielefeld University, Bielefeld, Germany., Busche T; Medical School East Westphalia-Lippe, Bielefeld University, Bielefeld, Germany., Kalinowski J; Technology Platform Genomics, Center for Biotechnology, Bielefeld University, Bielefeld, Germany., Zischka M; Institute for Instrumental Analytics and Bioanalytics, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Weller P; Institute for Instrumental Analytics and Bioanalytics, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany., Nouioui I; Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany., Neumann-Schaal M; Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany., Risdian C; Department of Microbial Strain Collection, Helmholtz Centre for Infection Research, Braunschweig, Germany.; Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Bandung, Indonesia., Wink J; Department of Microbial Strain Collection, Helmholtz Centre for Infection Research, Braunschweig, Germany.; German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany., Mack M; Institute for Technical Microbiology, Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany.
Jazyk: angličtina
Zdroj: Environmental microbiology reports [Environ Microbiol Rep] 2024 Apr; Vol. 16 (2), pp. e13266.
DOI: 10.1111/1758-2229.13266
Abstrakt: The Gram-positive bacteria Streptomyces davaonensis and Streptomyces cinnabarinus have been the only organisms known to produce roseoflavin, a riboflavin (vitamin B 2 ) derived red antibiotic. Using a selective growth medium and a phenotypic screening, we were able to isolate a novel roseoflavin producer from a German soil sample. The isolation procedure was repeated twice, that is, the same strain could be isolated from the same location in Berlin 6 months and 12 months after its first isolation. Whole genome sequencing of the novel roseoflavin producer revealed an unusual chromosomal arrangement and the deposited genome sequence of the new isolate (G + C content of 71.47%) contains 897 genes per inverted terminal repeat, 6190 genes in the core and 107 genes located on an illegitimate terminal end. We identified the roseoflavin biosynthetic genes rosA, rosB and rosC and an unusually high number of riboflavin biosynthetic genes. Overexpression of rosA, rosB and rosC in Escherichia coli and enzyme assays confirmed their predicted functions in roseoflavin biosynthesis. A full taxonomic analysis revealed that the isolate represents a previously unknown Streptomyces species and we propose the name Streptomyces berlinensis sp. nov. for this roseoflavin producer.
(© 2024 The Authors. Environmental Microbiology Reports published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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