Eugenia uniflora (pitanga) juice as a new alternative vehicle for Limosilactobacillus fermentum ATCC 23271: evaluation of antioxidant and anti-infective effects.

Autor: Costa Dos Santos GD; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., da Silva Pereira CD; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., da Silva CC; Laboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., Silva MA; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., Silva LDS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., da Silva Cutrim B; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil., Branco SJDSC; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., Paiva PMG; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil., Correia MTDS; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil., Miranda RCM; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., Zagmignan A; Laboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, 65075-120, MA, Brazil., Nascimento da Silva LC; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, 65075-120, MA, Brazil. luiscn.silva@ceuma.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1279-1286. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1007/s42770-024-01329-7
Abstrakt: Probiotic-containing foods are among the most appreciated functional foods; however, probiotic-based dairy products cannot be consumed by people who are lactose intolerant, allergic to milk, or vegetarian or vegan individuals. Thus, new non-dairy matrices have been tested for probiotics delivery. This study evaluated the growth and viability of Limosilactobacillus fermentum ATCC 23271 and Lacticaseibacillus rhamnosus ATCC 9595 in Pitanga juice (Eugenia uniflora L.). The effects of the fermentation on the antioxidant and anti-infective properties of the juice were also analyzed. The E. uniflora juice allowed lactobacilli growth without supplementation, reaching rates around 8.4 Log CFU/mL and producing organic acids (pH values < 4) after 72 h of fermentation. The strain remained viable after 35 days of refrigerated storage. Fermentation by these bacteria increases the antioxidant capacity of the juice. The central composite rotational design was employed to evaluate the effects of bacterial inoculum and pulp concentration on growth and organic acids production by L. fermentum ATCC 23271. The strain was viable and produced organic acids in all tested combinations. L. fermentum-fermented juice and its cell-free supernatant significantly increased the survival of Tenebrio molitor larvae infected by enteroaggregative Escherichia coli 042. The results obtained in this study provide more insights into the potential of Pitanga juice to develop a functional non-dairy probiotic beverage with antioxidant and anti-infective properties.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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