UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope.

Autor:	Liao Y; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Andronov L; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France.; Department of Integrated Structural Biology, Centre for Integrative Biology, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France., Liu X; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Lin J; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Guerber L; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Lu L; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Agote-Arán A; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Pangou E; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Ran L; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Kleiss C; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Qu M; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Schmucker S; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Cirillo L; Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.; iGE3 Institute of Genetics and Genomics of Geneva , Geneva, Switzerland., Zhang Z; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Riveline D; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France., Gotta M; Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.; iGE3 Institute of Genetics and Genomics of Geneva , Geneva, Switzerland., Klaholz BP; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France.; Department of Integrated Structural Biology, Centre for Integrative Biology, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France., Sumara I; Department of Development and Stem Cells, Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.; Centre National de la Recherche Scientifique UMR 7104 , Strasbourg, France.; Institut National de la Santé et de la Recherche Médicale U964 , Strasbourg, France.; Université de Strasbourg , Strasbourg, France.
Jazyk:	angličtina
Zdroj:	The Journal of cell biology [J Cell Biol] 2024 Jul 01; Vol. 223 (7). Date of Electronic Publication: 2024 Apr 23.
DOI:	10.1083/jcb.202310006
Abstrakt:	Assembly of macromolecular complexes at correct cellular sites is crucial for cell function. Nuclear pore complexes (NPCs) are large cylindrical assemblies with eightfold rotational symmetry, built through hierarchical binding of nucleoporins (Nups) forming distinct subcomplexes. Here, we uncover a role of ubiquitin-associated protein 2-like (UBAP2L) in the assembly and stability of properly organized and functional NPCs at the intact nuclear envelope (NE) in human cells. UBAP2L localizes to the nuclear pores and facilitates the formation of the Y-complex, an essential scaffold component of the NPC, and its localization to the NE. UBAP2L promotes the interaction of the Y-complex with POM121 and Nup153, the critical upstream factors in a well-defined sequential order of Nups assembly onto NE during interphase. Timely localization of the cytoplasmic Nup transport factor fragile X-related protein 1 (FXR1) to the NE and its interaction with the Y-complex are likewise dependent on UBAP2L. Thus, this NPC biogenesis mechanism integrates the cytoplasmic and the nuclear NPC assembly signals and ensures efficient nuclear transport, adaptation to nutrient stress, and cellular proliferative capacity, highlighting the importance of NPC homeostasis at the intact NE. (© 2024 Liao et al.)
Databáze:	MEDLINE
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