Exercise Training Attenuates the Muscle Mitochondria Genomic Response to Bed Rest.

Autor:	Cotter JA, Plaza-Florido A; Pediatric Exercise and Genomics Research Center, School of Medicine, University of California, Irvine, CA., Adams GR; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA., Haddad F; Pediatric Exercise and Genomics Research Center, School of Medicine, University of California, Irvine, CA., Scott JM, Everett M; National Aeronautics and Space Administration (NASA), Houston, TX., Ploutz-Snyder L; School of Kinesiology, University of Michigan, Ann Arbor, MI., Radom-Aizik S; Pediatric Exercise and Genomics Research Center, School of Medicine, University of California, Irvine, CA.
Jazyk:	angličtina
Zdroj:	Medicine and science in sports and exercise [Med Sci Sports Exerc] 2024 Sep 01; Vol. 56 (9), pp. 1615-1622. Date of Electronic Publication: 2024 Apr 23.
DOI:	10.1249/MSS.0000000000003457
Abstrakt:	Purpose: Exercise training during the National Aeronautics and Space Administration 70-d bed rest study effectively counteracted the decline in aerobic capacity, muscle mass, strength, and endurance. We aimed to characterize the genomic response of the participants' vastus lateralis on day 64 of bed rest with and without exercise countermeasures. Methods: Twenty-two healthy young males were randomized into three groups: 1) bed rest only ( n = 7), 2) bed rest + aerobic (6 d·wk -1 ) and resistance training (3 d·wk -1 ) on standard equipment ( n = 7), and 3) bed rest + aerobic and resistance training using a flywheel device ( n = 8). The vastus lateralis gene and microRNA microarrays were analyzed using GeneSpring GX 14.9.1 (Agilent Technologies, Palo Alto, CA). Results: Bed rest significantly altered the expression of 2113 annotated genes in at least one out of the three study groups (fold change (FC) > 1.2; P < 0.05). Interaction analysis revealed that exercise attenuated the bed rest effect of 511 annotated genes (FC = 1.2, P < 0.05). In the bed rest only group, a predominant downregulation of genes was observed, whereas in the two exercise groups, there was a notable attenuation or reversal of this effect, with no significant differences between the two exercise modalities. Enrichment analysis identified functional categories and gene pathways, many of them related to the mitochondria. In addition, bed rest significantly altered the expression of 35 microRNAs (FC > 1.2, P < 0.05) with no difference between the three groups. Twelve are known to regulate some of the mitochondrial-related genes that were altered following bed rest. Conclusions: Mitochondrial gene expression was a significant component of the molecular response to long-term bed rest. Although exercise attenuated the FC in the downregulation of many genes, it did not completely counteract all the molecular consequences. (Copyright © 2024 by the American College of Sports Medicine.)
Databáze:	MEDLINE
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