Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing.

Autor: Lanszki Z; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary. lanszkizsofi@gmail.com.; Institute of Biology, Faculty of Sciences, University of Pécs, Pecs, Hungary. lanszkizsofi@gmail.com., Islam MS; Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh., Shikder MF; Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh., Sarder MJU; Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh., Khan SA; Department of Physiology Biochemistry and Pharmacology, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh., Chowdhury S; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, One Health Institute, Rajshahi, Bangladesh., Islam MN; Department of Forest and Wildlife Ecology, Wisconsin Cooperative Wildlife Research Unit, University of Wisconsin-Madison, Madison, USA., Tauber Z; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.; School of Biomedical Sciences, University of Plymouth, Plymouth, PL4 8AA, UK., Tóth GE; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pecs, Hungary., Jakab F; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pecs, Hungary., Kemenesi G; National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.; Institute of Biology, Faculty of Sciences, University of Pécs, Pecs, Hungary., Akter S; Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 22; Vol. 14 (1), pp. 9250. Date of Electronic Publication: 2024 Apr 22.
DOI: 10.1038/s41598-024-59343-6
Abstrakt: Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions.
(© 2024. The Author(s).)
Databáze: MEDLINE
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