Sourcing chitin from exoskeleton of Tenebrio molitor fed with polystyrene or plastic kitchen wrap.

Autor: Ilijin L; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia. Electronic address: lararid@ibiss.bg.ac.rs., Nikolić MV; University of Belgrade - Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade, Serbia., Vasiljević ZZ; University of Belgrade - Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade, Serbia., Todorović D; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia., Mrdaković M; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia., Vlahović M; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia., Matić D; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia., Tadić NB; University of Belgrade, Faculty of Physics, Studentski trg 12, 11000 Belgrade, Serbia., Perić-Mataruga V; Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, University of Belgrade, Despot Stefan Blv. 142, 11060 Belgrade, Serbia.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 268 (Pt 2), pp. 131731. Date of Electronic Publication: 2024 Apr 20.
DOI: 10.1016/j.ijbiomac.2024.131731
Abstrakt: In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1: 1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78-5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61 % was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of α-chitin with a crystallinity index of 66.5-62 % and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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