Nardilysin in vascular smooth muscle cells controls blood pressure via the regulation of calcium dynamics.

Autor: Batbaatar MA; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan; Department of Pharmacology, School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210, Mongolia., Kinoshita T; Division of Cardiovascular Surgery and Thoracic Surgery, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan., Ikeda S; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan., Nishi K; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan., Iwasaki H; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan., Ganbaatar N; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan., Ohno M; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan. Electronic address: moono@belle.shiga-med.ac.jp., Nishi E; Department of Pharmacology, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan. Electronic address: enishi@belle.shiga-med.ac.jp.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Jun 18; Vol. 712-713, pp. 149961. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1016/j.bbrc.2024.149961
Abstrakt: Blood pressure is a crucial physiological parameter and its abnormalities can cause a variety of health problems. We have previously reported that mice with systemic deletion of nardilysin (NRDC), an M16 family metalloprotease, exhibit hypotension. In this study, we aimed to clarify the role of NRDC in vascular smooth muscle cell (VSMC) by generating VSMC-specific Nrdc knockout (VSMC-KO) mice. Our findings reveal that VSMC-KO mice also exhibit hypotension. Aortas isolated from VSMC-KO mice exhibited a weakened contractile response to phenylephrine, accompanied by reduced phosphorylation of myosin light chain 2 and decreased rhoA expression. VSMC isolated from VSMC-KO aortas showed a reduced increase in intracellular Ca 2+ concentration induced by α-stimulants. These findings suggest that NRDC in VSMC regulates vascular contraction and blood pressure by modulating Ca 2+ dynamics.
Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest with the contents of this article.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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