Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish.

Autor: Chen X; School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, People's Republic of China., Chen J; Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People's Republic of China., Wang S; Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People's Republic of China., Yu S; School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, People's Republic of China., Liu Z; Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People's Republic of China. lzg@jlict.edu.cn., Zeng X; Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People's Republic of China. jiangzxd@163.com.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2024 Apr 22. Date of Electronic Publication: 2024 Apr 22.
DOI: 10.1007/s10895-024-03642-8
Abstrakt: We have prepared a simple, universal and efficient coumarin-derived fluorescent probe (XDS1) to detecting HOCl. The experimental findings revealed that the introduction of HOCl produced an obvious quenching effect on the probe with high selectivity and sensitivity. The calculated limit of detection (LOD) was as low as 0.02 μM. Furthermore, an impressive response time of less than 10 s was observed when XDS1 detecting HOCl. Importantly, the probe XDS1 exhibited negligible cytotoxicity, thereby facilitating its application for imaging HOCl within biological environment. The probe XDS1 had been successfully used for specific detection in cells.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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