Sulphite addition during steam pretreatment enhanced both enzyme-mediated cellulose hydrolysis and ethanol production.

Autor: Zhong N; Department of Wood Science, Faculty of Forestry, Forest Products Biotechnology and Bioenergy Group, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada.; St1 Oy, Firdonkatu 2, Helsinki, Finland., Chandra R; Trinity Western University, 22500 University Dr, Langley, BC, Canada. richard.chandra@twu.ca., Yamamoto M; St1 Oy, Firdonkatu 2, Helsinki, Finland., Leskinen T; St1 Oy, Firdonkatu 2, Helsinki, Finland., Granström T; VTT Technical Research Centre of Finland Ltd., 02044, Espoo, Finland., Saddler J; Department of Wood Science, Faculty of Forestry, Forest Products Biotechnology and Bioenergy Group, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada. jack.saddler@ubc.ca.
Jazyk: angličtina
Zdroj: Bioresources and bioprocessing [Bioresour Bioprocess] 2022 Jun 29; Vol. 9 (1), pp. 71. Date of Electronic Publication: 2022 Jun 29.
DOI: 10.1186/s40643-022-00556-w
Abstrakt: Sulphite addition during steam pretreatment of softwoods under acidic, neutral and alkaline conditions was assessed to try to minimize lignin condensation. Although pretreatment under neutral/alkaline conditions resulted in effective lignin sulphonation, non-uniform size reduction was observed. In contrast, acidic sulphite steam treatment at 210 °C for 10 min resulted in homogenous particle size reduction and water-insoluble component that was 62% carbohydrate and 33% lignin. This carbohydrate-rich substrate was readily hydrolyzed and fermented which indicated the lack of fermentation inhibitors in the steam-pretreated whole slurry. The use of high solid loading (25% w/v) resulted in a hydrolysis yield of 58% at an enzyme loading of 40 mg protein/g glucan and efficient fermentation (46.6 g/L of ethanol). This indicated that the addition of acidic sulphite at the steam pretreatment of softwoods improved both the enzymatic hydrolysis and fermentation of steam-pretreated whole slurries.
(© 2022. The Author(s).)
Databáze: MEDLINE
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