Viewing early life without labels: optical approaches for imaging the early embryo†.

Autor:	Chow DJX; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.; Centre of Light for Life, The University of Adelaide, Adelaide, Australia., Tan TCY; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia., Upadhya A; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.; Centre of Light for Life, The University of Adelaide, Adelaide, Australia.; School of Biological Sciences, The University of Adelaide, Adelaide, Australia., Lim M; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.; Centre of Light for Life, The University of Adelaide, Adelaide, Australia.; School of Biological Sciences, The University of Adelaide, Adelaide, Australia., Dholakia K; Centre of Light for Life, The University of Adelaide, Adelaide, Australia.; School of Biological Sciences, The University of Adelaide, Adelaide, Australia.; Scottish Universities Physics Alliance, School of Physics and Astronomy, University of St Andrews, St Andrews, United Kingdom., Dunning KR; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.; Centre of Light for Life, The University of Adelaide, Adelaide, Australia.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2024 Jun 12; Vol. 110 (6), pp. 1157-1174.
DOI:	10.1093/biolre/ioae062
Abstrakt:	Embryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo quality rely on subjective morphology assessments or an invasive biopsy for genetic testing. However, both approaches can be inherently inaccurate and crucially, fail to improve the live birth rate following the transfer of in vitro produced embryos. Optical imaging offers a potential non-invasive and accurate avenue for assessing embryo viability. Recent advances in various label-free optical imaging approaches have garnered increased interest in the field of reproductive biology due to their ability to rapidly capture images at high resolution, delivering both morphological and molecular information. This burgeoning field holds immense potential for further development, with profound implications for clinical translation. Here, our review aims to: (1) describe the principles of various imaging systems, distinguishing between approaches that capture morphological and molecular information, (2) highlight the recent application of these technologies in the field of reproductive biology, and (3) assess their respective merits and limitations concerning the capacity to evaluate embryo quality. Additionally, the review summarizes challenges in the translation of optical imaging systems into routine clinical practice, providing recommendations for their future development. Finally, we identify suitable imaging approaches for interrogating the mechanisms underpinning successful embryo development. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction.)
Databáze:	MEDLINE
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