The integrated on-chip isolation and detection of circulating tumour cells.

Autor:	Abusamra SM; Nuffield Department of Surgical Sciences, University of Oxford Oxford OX3 9DU UK., Barber R; Department of Chemistry, University of Oxford Oxford OX1 3QZ UK jason.davis@chem.ox.ac.uk., Sharafeldin M; Department of Chemistry, University of Otago Dunedin 9016 New Zealand., Edwards CM; Nuffield Department of Surgical Sciences, University of Oxford Oxford OX3 9DU UK.; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Systems, University of Oxford Oxford UK., Davis JJ; Department of Chemistry, University of Oxford Oxford OX1 3QZ UK jason.davis@chem.ox.ac.uk.
Jazyk:	angličtina
Zdroj:	Sensors & diagnostics [Sens Diagn] 2024 Mar 26; Vol. 3 (4), pp. 562-584. Date of Electronic Publication: 2024 Mar 26 (Print Publication: 2024).
DOI:	10.1039/d3sd00302g
Abstrakt:	Circulating tumour cells (CTCs) are cancer cells shed from a primary tumour which intravasate into the blood stream and have the potential to extravasate into distant tissues, seeding metastatic lesions. As such, they can offer important insight into cancer progression with their presence generally associated with a poor prognosis. The detection and enumeration of CTCs is, therefore, critical to guiding clinical decisions during treatment and providing information on disease state. CTC isolation has been investigated using a plethora of methodologies, of which immunomagnetic capture and microfluidic size-based filtration are the most impactful to date. However, the isolation and detection of CTCs from whole blood comes with many technical barriers, such as those presented by the phenotypic heterogeneity of cell surface markers, with morphological similarity to healthy blood cells, and their low relative abundance (∼1 CTC/1 billion blood cells). At present, the majority of reported methods dissociate CTC isolation from detection, a workflow which undoubtedly contributes to loss from an already sparse population. This review focuses on developments wherein isolation and detection have been integrated into a single-step, microfluidic configuration, reducing CTC loss, increasing throughput, and enabling an on-chip CTC analysis with minimal operator intervention. Particular attention is given to immune-affinity, microfluidic CTC isolation, coupled to optical, physical, and electrochemical CTC detection (quantitative or otherwise). Competing Interests: There are no conflicts of interest to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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