Neisseria meningitidis with decreased susceptibility to penicillin G and molecular characterization of the penA gene in strains isolated at University Hospital Centers of Casablanca and Marrakech (Morocco).

Autor:	Mouss KA; Department of Microbiology, Laboratory of Clinical Immunology, Inflammation and Allergy, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca, Morocco.; Bacteriology-Virology and Hospital Hygiene Laboratory, University Hospital Centre Ibn Rochd, 1 Rue des Hôpitaux, 20100, Casablanca, Morocco.; Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Morocco., Nzoyikorera N; Department of Microbiology, Laboratory of Clinical Immunology, Inflammation and Allergy, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca, Morocco., Razki A; Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Morocco., Zaki B; Bacteriology-Virology and Hospital Hygiene Laboratory, University Hospital Centre Ibn Rochd, 1 Rue des Hôpitaux, 20100, Casablanca, Morocco., Soraa N; Faculty of Medicine and Pharmacy of Marrakech Cadi Ayyad University, Microbiology Department, Mohamed VI University Hospital Center, Marrakech, Morocco., Zerouali K; Department of Microbiology, Laboratory of Clinical Immunology, Inflammation and Allergy, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca, Morocco.; Bacteriology-Virology and Hospital Hygiene Laboratory, University Hospital Centre Ibn Rochd, 1 Rue des Hôpitaux, 20100, Casablanca, Morocco.
Jazyk:	angličtina
Zdroj:	The Pan African medical journal [Pan Afr Med J] 2024 Feb 08; Vol. 47, pp. 56. Date of Electronic Publication: 2024 Feb 08 (Print Publication: 2024).
DOI:	10.11604/pamj.2024.47.56.42328
Abstrakt:	Introduction: the laboratory diagnosis of meningococcal meningitis relies on conventional techniques. This study aims to evaluate the correlation between the reduced sensitivity to penicillin G of Neisseria meningitidis (N.m) strains and the expression of the altered PBP 2 gene. Methods: out of 190 strains of N.m isolated between 2010 and 2021 at the bacteriology laboratories of Ibn Rochd University Hospital Centre (IR-UHC) in Casablanca and the UHC Mohammed VI in Marrakech, 23 isolates were part of our study. We first determined their state of sensitivity to penicillin G by E-Test strips and searched for the expression of the penA gene by PCR followed by Sanger sequencing. Results: of all the confirmed cases of N.m, 93.15% (n=177) are of serogroup B, 75.2% (n = 143) are sensitive to penicillin G and 24.73% (n = 47) are of intermediate sensitivity. No resistance to penicillin G was observed. Reduced sensitivity to penicillin G in N.m is characterized by mutations namely F504 L, A510 V, I515 V, G541 N and I566 V located in the C-terminal region of the penA gene encoding the penicillin-binding protein 2 (PBP2) (mosaic gene). Conclusion: our study presents useful data for the phenotypic and genotypic monitoring of resistance to penicillin G in N.m and can contribute to the analysis of genetic exchanges between different Neisseria species. Competing Interests: The authors declare no competing interests. (Copyright: Khadija Ait Mouss et al.)
Databáze:	MEDLINE
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