Multi-scale in vivo imaging of tumour development using a germline conditional triple-reporter system.

Autor:	Dzien P; Cancer Research UK Scotland Institute., Raffo Iraolagoitia X; Cancer Research UK Scotland Institute., May S; Cancer Research UK Scotland Institute., Stevenson D; Cancer Research UK Scotland Institute., McGarry L; Cancer Research UK Scotland Institute., Soloviev D; Cancer Research UK Scotland Institute., Brown G; Cancer Research UK Scotland Institute., Nixon C; Cancer Research UK Scotland Institute., Kapeni C; Cancer Research UK Cambridge Institute., De La Roche M; Cancer Research UK Cambridge Institute., Blyth K; CRUK Scotland Institute., Lyons S; Cold Spring Harbor Laboratory., Bird T; Cancer Research UK Scotland Institute., Strathdee D; Cancer Research UK Scotland Institute., Fruhwirth G; Kings College London., Carlin L; Cancer Research UK Scotland Institute., Lewis D; Cancer Research UK Scotland Institute.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2024 Apr 03. Date of Electronic Publication: 2024 Apr 03.
DOI:	10.21203/rs.3.rs-4196140/v1
Abstrakt:	Imaging reporter genes are indispensable for visualising biological processes in living subjects, particularly in cancer research where they have been used to observe tumour development, cancer cell dissemination, and treatment response. Engineering reporter genes into the germline frequently involves single imaging modality reporters operating over limited spatial scales. To address these limitations, we developed an inducible triple-reporter mouse model (Rosa26 LSL - NRL ) that integrates reporters for complementary imaging modalities, flfluorescence, bioluminescence and positron emission tomography (PET), along with inducible Cre-lox functionality for precise spatiotemporal control of reporter expression. We demonstrated robust reporter inducibility across various tissues in the Rosa26 LSL - NRL mouse, facilitating effective tracking and characterisation of tumours in liver and lung cancer mouse models. We precisely pinpointed tumour location using multimodal whole-body imaging which guided in situ lung microscopy to visualise cell-cell interactions within the tumour microenvironment. The triple-reporter system establishes a robust new platform technology for multi-scale investigation of biological processes within whole animals, enabling tissue-specific and sensitive cell tracking, spanning from the whole-body to cellular scales. Competing Interests: Additional Declarations: There is NO Competing Interest.
Databáze:	MEDLINE
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