Characterizing Prion-Like Protein Aggregation: Emerging Nanopore-Based Approaches.

Autor: Meyer N; Institut Européen des Membranes, UMR5635 University of Montpellier ENCSM CNRS, Place Eugène Bataillon, Cedex 5, Montpellier, 34095, France.; INM, University of Montpellier, INSERM, Montpellier, 34095, France., Torrent J; INM, University of Montpellier, INSERM, Montpellier, 34095, France., Balme S; Institut Européen des Membranes, UMR5635 University of Montpellier ENCSM CNRS, Place Eugène Bataillon, Cedex 5, Montpellier, 34095, France.
Jazyk: angličtina
Zdroj: Small methods [Small Methods] 2024 Apr 21, pp. e2400058. Date of Electronic Publication: 2024 Apr 21.
DOI: 10.1002/smtd.202400058
Abstrakt: Prion-like protein aggregation is characteristic of numerous neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases. This process involves the formation of aggregates ranging from small and potentially neurotoxic oligomers to highly structured self-propagating amyloid fibrils. Various approaches are used to study protein aggregation, but they do not always provide continuous information on the polymorphic, transient, and heterogeneous species formed. This review provides an updated state-of-the-art approach to the detection and characterization of a wide range of protein aggregates using nanopore technology. For each type of nanopore, biological, solid-state polymer, and nanopipette, discuss the main achievements for the detection of protein aggregates as well as the significant contributions to the understanding of protein aggregation and diagnostics.
(© 2024 The Authors. Small Methods published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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