| Autor: |
Ćorović K; Community Health Center Tutin, Department of Emergency Medicine, Bogoljuba Čukića 12, 36320 Tutin, Republic of Serbia.; University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Stojković DL; University of Kragujevac, Institute for Information Technologies, Department of Science, Jovana Cvijića bb, 34000 Kragujevac, Republic of Serbia. danijela.stojkovic@kg.ac.rs., Petrović ĐS; University of Kragujevac, Faculty of Science, Department of Chemistry, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia., Jovičić Milić SS; University of Kragujevac, Institute for Information Technologies, Department of Science, Jovana Cvijića bb, 34000 Kragujevac, Republic of Serbia. danijela.stojkovic@kg.ac.rs., Đukić MB; University of Kragujevac, Faculty of Science, Department of Chemistry, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia., Radojević ID; University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia., Raković I; University of Kragujevac, Faculty of Medical Sciences, Department of Infectious Diseases, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Jurišević M; University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia.; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Gajović N; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Jovanović M; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia.; University of Kragujevac, Faculty of Medical Sciences, Department of Otorinolaringology, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Marinković J; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Jovanović I; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia., Stojanović B; University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia.; University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Svetozara Markovića 69, 34000 Kragujevac, Republic of Serbia. |
| Abstrakt: |
The four new ligands, dialkyl esters of ( S , S )-propylenediamine- N , N '-di-(2,2'-di-(4-hydroxy-benzil))acetic acid (R 2 - S , S -pddtyr·2HCl) (R = ethyl (L1), propyl (L2), butyl (L3), and pentyl (L4)) and corresponding palladium(II) complexes have been synthesized and characterized by microanalysis, infrared, 1 H NMR and 13 C NMR spectroscopy. In vitro cytotoxicity was evaluated using the MTT assay on four tumor cell lines, including mouse mammary (4T1) and colon (CT26), and human mammary (MDA-MD-468) and colon (HCT116), as well as non-tumor mouse mesenchymal stem cells. Using fluorescence spectroscopy were investigated the interactions of new palladium(II) complexes [PdCl 2 (R 2 - S , S -pddtyr)]; (R = ethyl (C1), propyl (C2), butyl (C3), and pentyl (C4)) with calf thymus human serum albumin (HSA) and DNA (CT-DNA). The high values of the binding constants, K b , and the Stern-Volmer quenching constant, K SV , show the good binding of all complexes for HSA and CT-DNA. The mentioned ligands and complexes were also tested on in vitro antimicrobial activity against 11 microorganisms. Testing was performed by the microdilution method, where the minimum inhibitory concentration (MMC) and the minimum microbicidal concentration (MMC) were determined. |
