Oxidative stress, inflammation, and steatosis elucidate the complex dynamics of HgCl 2 induced liver damage in Channa punctata.

Autor: Singh S; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India., Dwivedi S; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India., Khan AA; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India., Jain A; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India., Dwivedi S; Department of Zoology, Government Degree College, Haripur-Nihastha, Raebareli, 229208, India., Yadav KK; Department of Zoology, Government Degree College, Bakkha Kheda, Unnao, 209801, India., Dubey I; Department of Zoology, DBS College, Kanpur, Uttar Pradesh, 208006, India., Trivedi A; Department of Zoology, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, 243006, India., Trivedi SP; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India., Kumar M; Environmental Toxicology and Bioremediation Laboratory (ETBL), Department of Zoology, University of Lucknow, Lucknow, 226007, India. mk2016lu@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 22; Vol. 14 (1), pp. 9161. Date of Electronic Publication: 2024 Apr 22.
DOI: 10.1038/s41598-024-59917-4
Abstrakt: Water bodies are highly pollution-prone areas in which mercury (Hg) is considered as a major menace to aquatic organisms. However, the information about the toxicity of mercuric chloride (HgCl 2 ) in a vital organ such as the liver of fish is still inadequate. This study aimed to assess the impact of mercuric chloride (HgCl 2 ) exposure on the liver of Channa punctata fish over 15, 30, and 45 days, at two different concentrations (0.039 mg/L and 0.078 mg/L). Mercury is known to be a significant threat to aquatic life, and yet, information regarding its effects on fish liver remains limited. The results of this study demonstrate that exposure to HgCl 2 significantly increases oxidative stress markers, such as lipid peroxidation (LPO) and protein carbonyls (PC), as well as the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) in the fish. Additionally, the transcriptional and protein analysis of specific genes and molecules associated with necroptosis and inflammation, such as ABCG2, TNF α, Caspase 3, RIPK 3, IL-1β, Caspase-1, IL-18, and RIPK1, confirm the occurrence of necroptosis and inflammation in the liver. Histopathological and ultrastructural examinations of the liver tissue further reveal a significant presence of liver steatosis. Interestingly, the upregulation of PPARα suggests that the fish's body is actively responding to counteract the effects of liver steatosis. This study provides a comprehensive analysis of oxidative stress, biochemical changes, gene expression, protein profiles, and histological findings in the liver tissue of fish exposed to mercury pollution in freshwater environments.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje