Effects of environmental exposure to iron powder on healthy and elastase-exposed mice.

Autor: Galli TT; Faculdade de Medicina (FMUSP), São Paulo, Brazil., de Campos EC; Faculdade de Medicina (FMUSP), São Paulo, Brazil.; Hospital Sírio-Libanês, São Paulo, Brazil., do Nascimento Camargo L; Faculdade de Medicina (FMUSP), São Paulo, Brazil.; Hospital Sírio-Libanês, São Paulo, Brazil., Fukuzaki S; Faculdade de Medicina (FMUSP), São Paulo, Brazil.; Hospital Alemão Oswaldo Cruz, São Paulo, Brazil., Dos Santos TM; Faculdade de Medicina (FMUSP), São Paulo, Brazil.; Hospital Sírio-Libanês, São Paulo, Brazil., Hamaguchi SSS; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Bezerra SKM; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Silva FJA; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Rezende BG; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Dos Santos Lopes FTQ; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Olivo CR; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Saraiva-Romanholo BM; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Prado CM; Federal University of São Paulo (UNIFESP), São Paulo, Brazil., Leick EA; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Bourotte CLM; Institute of Geosciences - University of São Paulo (USP), São Paulo, Brazil., Benseñor IJM; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Lotufo PA; Faculdade de Medicina (FMUSP), São Paulo, Brazil., Righetti RF; Faculdade de Medicina (FMUSP), São Paulo, Brazil.; Hospital Sírio-Libanês, São Paulo, Brazil., Tibério IFLC; Faculdade de Medicina (FMUSP), São Paulo, Brazil. iocalvo@uol.com.br.; University of São Paulo, Av. Dr. Arnaldo, 455 - Cerqueira César, São Paulo, SP, 01246-903 - Laboratory LIM20, Brazil. iocalvo@uol.com.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 21; Vol. 14 (1), pp. 9134. Date of Electronic Publication: 2024 Apr 21.
DOI: 10.1038/s41598-024-59573-8
Abstrakt: Prolonged exposure to iron powder and other mineral dusts can threaten the health of individuals, especially those with COPD. The goal of this study was to determine how environmental exposure to metal dust from two different mining centers in Brazil affects lung mechanics, inflammation, remodeling and oxidative stress responses in healthy and elastase-exposed mice. This study divided 72 male C57Bl/6 mice into two groups, the summer group and the winter group. These groups were further divided into six groups: control, nonexposed (SAL); nonexposed, given elastase (ELA); exposed to metal powder at a mining company (SAL-L1 and ELA-L1); and exposed to a location three miles away from the mining company (SAL-L2 and ELA-L2) for four weeks. On the 29th day of the protocol, the researchers assessed lung mechanics, bronchoalveolar lavage fluid (BALF), inflammation, remodeling, oxidative stress, macrophage iron and alveolar wall alterations (mean linear intercept-Lm). The Lm was increased in the ELA, ELA-L1 and ELA-L2 groups compared to the SAL group (p < 0.05). There was an increase in the total number of cells and macrophages in the ELA-L1 and ELA-L2 groups compared to the other groups (p < 0.05). Compared to the ELA and SAL groups, the exposed groups (ELA-L1, ELA-L2, SAL-L1, and SAL-L2) exhibited increased expression of IL-1β, IL-6, IL-10, IL-17, TNF-α, neutrophil elastase, TIMP-1, MMP-9, MMP-12, TGF-β, collagen fibers, MUC5AC, iNOS, Gp91phox, NFkB and iron positive macrophages (p < 0.05). Although we did not find differences in lung mechanics across all groups, there were low to moderate correlations between inflammation remodeling, oxidative stress and NFkB with elastance, resistance of lung tissue and iron positive macrophages (p < 0.05). Environmental exposure to iron, confirmed by evaluation of iron in alveolar macrophages and in air, exacerbated inflammation, initiated remodeling, and induced oxidative stress responses in exposed mice with and without emphysema. Activation of the iNOS, Gp91phox and NFkB pathways play a role in these changes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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