Novel RP-HPLC method for estimation of a newly developed combination of tizanidine and etoricoxib in rat plasma: Eight criteria for greens evaluation.

Autor: Assirey EA; Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah, Madinah, Saudi Arabia., Ahmed HA; Chemistry Department, Faculty of Science at Yanbu, Taibah University, Yanbu, Saudi Arabia.; Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt., Al-Faze R; Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah, Madinah, Saudi Arabia., Amin MS; Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah, Madinah, Saudi Arabia.; Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Mahgoub SM; Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt., Abo El-Ela FI; Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt., Mohamed MA; Hikma Pharmaceutical Company, Beni-Suef, Egypt.
Jazyk: angličtina
Zdroj: Archiv der Pharmazie [Arch Pharm (Weinheim)] 2024 Aug; Vol. 357 (8), pp. e2400025. Date of Electronic Publication: 2024 Apr 21.
DOI: 10.1002/ardp.202400025
Abstrakt: A novel environmentally friendly reversed-phase high-performance liquid chromatography (RP-HPLC) method has been effectively validated for simultaneously measuring a prospective conjunction of tizanidine (TIZ) and etoricoxib (ETC), the combined medicine, in rat plasma. The technique employs diclofenac potassium as the internal standard, guaranteeing dependable and precise outcomes. This study aimed to assess the impact of the suggested combination therapy on treating inflammation resulting from rheumatoid arthritis (RA) in a rat model. The procedure was performed using an Agilent series 1200 model HPLC apparatus. The chromatographic conditions consist of isocratic elution mode, C18 column with dimensions of 150 mm × 4.6 mm × 5 µm, flow rate of 1.5 mL/min, wavelength of 230 nm, temperature of 50°C, and injection volume of 10 µL. The elution was performed using a mobile phase consisting of a phosphate buffer with a pH of 3.5 and acetonitrile in a ratio of 80:20 v/v. Calibration curves were conducted for TIZ and ETC within the 1-50 µg/mL range, demonstrating linear trends with R 2 values over 0.999. The effectiveness and eco-friendliness of the proposed method were evaluated using eight separate environmentally conscious metrics. The addition of TIZ and ETC to arthritic rodents amplified these effects significantly. Furthermore, TIZ and ETC significantly reduced serum levels in arthritic rodents, and safety investigations revealed normal complete blood count, liver, and renal functions. TIZ and ETC appear to have antiarthritic, anti-inflammatory, and safe combinations, making them viable future treatment options for RA that are also safe and efficacious. Following validation by United States Food and Drug Administration (US-FDA) rules, all goods met the criteria.
(© 2024 Deutsche Pharmazeutische Gesellschaft.)
Databáze: MEDLINE
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