Moringin, an isothiocyanate modulates multiple cellular signalling molecules in breast cancer cells.

Autor: Srivastava A; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India., Mishra S; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India., Avadhesh; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India., Shekher A; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India; Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India., Rai V; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India., Dhasmana A; Department of Bioscience and Cancer Research Institute, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun 248 016, India; Department of Immunology and Microbiology, School of Medicine, University of Texas Rio Grande Valley, Edinburg, TX, USA., Das J; Department of Biochemistry, All India Institute of Medical Sciences, Guwahati, India., Perenzoni D; Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy., Iori R; Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy., Gupta SC; Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221 005, India; Department of Biochemistry, All India Institute of Medical Sciences, Guwahati, India. Electronic address: sgupta@bhu.ac.in.
Jazyk: angličtina
Zdroj: Cellular signalling [Cell Signal] 2024 Jul; Vol. 119, pp. 111181. Date of Electronic Publication: 2024 Apr 21.
DOI: 10.1016/j.cellsig.2024.111181
Abstrakt: Prohibitin (PHB) is a pleiotropic molecule with a variety of known functions and subcellular locations. PHB's function in breast cancer is poorly understood. Herein, we report that PHB is expressed in cancer types of diverse origin including breast cancer. The cancer patients with changes in PHB were reported to have significantly reduced 'overall survival' in comparison to the cases without alterations in PHB. The expression of PHB was increased by H 2 O 2 and also by Moringin (MG), which is an isothiocyanate derived from the seeds of Moringa oleifera. MG interacted with PHB, DRP1, and SLP2 and inhibited the growth of MCF-7 and MDAMB-231 cells. The isothiocyanate triggered apoptosis in breast cancer cells as revealed by AO/PI assay, phosphatidylserine externalization, cell cycle analysis and DAPI staining. MG induced proapoptotic proteins expression such as cytochrome c, p53, and cleaved caspase-7. Further, cell survival proteins such as survivin, Bcl-2, and Bcl-xL were suppressed. A depolarization of membrane potential suggested that the apoptosis was triggered through mitochondria. The isothiocyanate suppressed the cancer cell migration and interacted with NF-κB subunits. MG suppressed p65 nuclear translocation induced by TNF-α. The reactive oxygen species generation was also induced by the isothiocyanate in breast cancer cells. MG also modulated the expression of lncRNAs. Collectively, the functions of PHB in breast cancer growth is evident from this study. The activities of MG against breast cancer might result from its ability to modulate multiple cancer-related targets.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: