Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention.

Autor: Gadaime NK; School of Medicine, The University of Jordan, Amman, Jordan.; Department of Lab Medicine, Faculty of Medical Technology-Derna, National Board for technical and Vocational Education, Derna, Libya., Haddadin RN; School of Pharmacy, The University of Jordan, Amman, Jordan., Shehabi AA; School of Medicine, The University of Jordan, Amman, Jordan., Omran IN; Faculty of Medicine, University of Benghazi, Benghazi, Libya.
Jazyk: angličtina
Zdroj: The Libyan journal of medicine [Libyan J Med] 2024 Dec 31; Vol. 19 (1), pp. 2344320. Date of Electronic Publication: 2024 Apr 21.
DOI: 10.1080/19932820.2024.2344320
Abstrakt: Pseudomonas aeruginosa is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms' capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes ( bla CTXM , bla TEM , bla SHV-1 , bla GES-1 , bla KPC , and bla NDM ) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while alginate production was measured spectrophotometrically. Resistance to antipseudomonal antibiotics ranged from 48.9% to 75.6%. The most prevalent resistance gene was blaNDM (26.7%), followed by blaGES-1 (17.8%). Moreover, all isolates demonstrated varying degrees of biofilm-forming ability and alginate production. No statistically significant correlation was found between biofilm formation and alginate production. The dissemination of resistant genes in P. aeruginosa, particularly carbapenemases, is of great concern. This issue is compounded by the bacteria's biofilm-forming capability. Urgent intervention and continuous surveillance are imperative to prevent further deterioration and the catastrophic spread of resistance among these formidable bacteria.
Databáze: MEDLINE
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