A high-precision wound healing assay based on photosensitized culture substrates.
Autor: | Azzam S; Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany., Tomasova L; Ibidi GmbH, Lochhamer Schlag 11, 82166, Graefelfing, Germany., Danner C; Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany., Skiba M; Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany., Klein M; Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany., Guttenberg Z; Ibidi GmbH, Lochhamer Schlag 11, 82166, Graefelfing, Germany., Michaelis S; Fraunhofer-Institut Fuer Elektronische Mikrosysteme Und Festkoerper-Technologien EMFT, Universitaetsstr. 31, 93053, Regensburg, Germany., Wegener J; Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany. Joachim.Wegener@ur.de.; Fraunhofer-Institut Fuer Elektronische Mikrosysteme Und Festkoerper-Technologien EMFT, Universitaetsstr. 31, 93053, Regensburg, Germany. Joachim.Wegener@ur.de. |
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Jazyk: | angličtina |
Zdroj: | Scientific reports [Sci Rep] 2024 Apr 20; Vol. 14 (1), pp. 9103. Date of Electronic Publication: 2024 Apr 20. |
DOI: | 10.1038/s41598-024-59564-9 |
Abstrakt: | Quantitative assessment of cell migration in vitro is often required in fundamental and applied research from different biomedical areas including wound repair, tumor metastasis or developmental biology. A collection of assays has been established throughout the years like the most widely used scratch assay or the so-called barrier assay. It is the principle of these assays to introduce a lesion into an otherwise confluent monolayer in order to study the migration of cells from the periphery into this artificial wound and determine the migration rate from the time necessary for wound closure. A novel assay makes use of photosensitizers doped into a polystyrene matrix. A thin layer of this composite material is coated on the bottom of regular cell culture ware showing perfect biocompatibility. When adherent cells are grown on this coating, resonant excitation of the photosensitizer induces a very local generation of 1 O (© 2024. The Author(s).) |
Databáze: | MEDLINE |
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