Generation of Anterior Segment of the Eye Cells from hiPSCs in Microfluidic Platforms.

Autor: Koçak G; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Uyulgan S; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Polatlı E; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Sarı V; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Kahveci B; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Bursali A; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye., Binokay L; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Reçber T; Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Sıhhiye, Ankara, 06100, Türkiye., Nemutlu E; Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Sıhhiye, Ankara, 06100, Türkiye., Mardinoğlu A; Science for Life Laboratory, KTH - Royal Institute of Technology, Stockholm, Sweden.; Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, London, SE1 9RT, UK., Karakülah G; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye., Utine CA; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Department of Ophthalmology, Dokuz Eylül University Hospital, Dokuz Eylül University, Izmir, 35340, Türkiye., Güven S; Izmir Biomedicine and Genome Center, Izmir, 35340, Türkiye.; Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Izmir, 35340, Türkiye.; Department of Medical Biology and Genetics, Faculty of Medicine, Dokuz Eylül University, Izmir, 35340, Türkiye.
Jazyk: angličtina
Zdroj: Advanced biology [Adv Biol (Weinh)] 2024 May; Vol. 8 (5), pp. e2400018. Date of Electronic Publication: 2024 Apr 19.
DOI: 10.1002/adbi.202400018
Abstrakt: Ophthalmic diseases affect many people, causing partial or total loss of vision and a reduced quality of life. The anterior segment of the eye accounts for nearly half of all visual impairment that can lead to blindness. Therefore, there is a growing demand for ocular research and regenerative medicine that specifically targets the anterior segment to improve vision quality. This study aims to generate a microfluidic platform for investigating the formation of the anterior segment of the eye derived from human induced pluripotent stem cells (hiPSC) under various spatial-mechanoresponsive conditions. Microfluidic platforms are developed to examine the effects of dynamic conditions on the generation of hiPSCs-derived ocular organoids. The differentiation protocol is validated, and mechanoresponsive genes are identified through transcriptomic analysis. Several culture strategies is implemented for the anterior segment of eye cells in a microfluidic chip. hiPSC-derived cells showed anterior eye cell characteristics in mRNA and protein expression levels under dynamic culture conditions. The expression levels of yes-associated protein and transcriptional coactivator PDZ binding motif (YAP/TAZ) and PIEZO1, varied depending on the differentiation and growth conditions of the cells, as well as the metabolomic profiles under dynamic culture conditions.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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