Ultrasensitive upconverting nanoprobes for in situ imaging of drug-induced liver injury using miR-122 as the biomarker.

Autor: Wang QL; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China., Meng LC; Institute of Chinese Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University, Nanjing, China., Zhao Z; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China., Du JF; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China., Li P; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China., Jiang Y; College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China. Electronic address: jiangyancpu@126.com., Li HJ; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: cpuli@163.com.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Jul 01; Vol. 274, pp. 126108. Date of Electronic Publication: 2024 Apr 18.
DOI: 10.1016/j.talanta.2024.126108
Abstrakt: Drug-induced liver injury (DILI) is a frequent adverse drug reaction. The current clinical diagnostic methods are inadequate for accurate and early detection of DILI due to the lack of effective diagnostic biomarkers. Hepatocyte-specific miR-122 is released from injured hepatocytes promptly and its efflux is significantly correlated with the progression of DILI. Therefore, achieving precise in situ detection of miR-122 with high sensitivity is vital for early visualization of DILI. Herein, a new nanoprobe, consisting of miR-122 aptamer, upconversion nanoparticles (UCNPs) and Prussian blue nanoparticles (PBNPs) was introduced for the early and sensitive detection of DILI in situ. As the nanoprobes reached in the liver, miR-122 aptamer-based entropy-driven strand displacement (ESDR) signal amplification reaction was triggered and luminescence resonance energy transfer (LRET) between UCNPs and PBNPs was responded to achieve the high-fidelity detection of DILI. A negative correlation was observed between the intensity of upconversion luminescence (UCL) and the concentration of miR-122. UCL imaging conducted both in vivo and ex vivo indicated that a reduction in miR-122 concentration led to an increase in UCL intensity, revealing a precise state of DILI. The detection technique demonstrated a positive correlation between signal intensity and severity, offering a more straightforward and intuitive method of visualizing DILI.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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