Spilanthes filicaulis (Schumach. & Thonn.) C.D. Adams leaves protects against streptozotocin-induced diabetic nephropathy.

Autor: Ojo OA; Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo, Nigeria., Ogunlakin AD; Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo, Nigeria., Akintayo CO; Department of Physiology, Afe Babalola University, Ado-Ekiti, Nigeria., Olukiran OS; Department of Physiology, Obafemi Awolowo University, Ile-Ife, Nigeria., Adetunji JB; Department of Biochemistry, Osun State University, Osogbo, Nigeria., Ajayi-Odoko OA; Department of Microbiology, Bowen University, Iwo, Nigeria., Ogwa TO; Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo, Nigeria., Molehin OR; Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria., Ojo OO; Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria., Mothana RA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi, Arabia., Alanzi AR; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi, Arabia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 19; Vol. 19 (4), pp. e0301992. Date of Electronic Publication: 2024 Apr 19 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0301992
Abstrakt: Background and Objective: Diabetic neuropathy (DN) is a complex type of diabetes. The underlying cause of diabetic nephropathy remains unclear and may be due to a variety of pathological conditions resulting in kidney failure. This study examines the protective effect of the methanolic extract of Spilanthes filicaulis leaves (MESFL) in fructose-fed streptozotocin (STZ)-induced diabetic nephropathy and the associated pathway.
Methods: Twenty-five rats were equally divided randomly into five categories: Control (C), diabetic control, diabetic + metformin (100 mg/kg), diabetic + MESFL 150 mg/kg bw, and diabetic + MESFL 300 mg/kg bw. After 15 days, the rats were evaluated for fasting blood glucose (FBG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, uric acid, serum creatinine, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation (MDA). Gene expression levels of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP response element-binding (CREB), cFOS and the antiapoptotic protein Bcl-2 were examined.
Results: We observed that MESFL at 150 and 300 mg/kg bw significantly downregulated the protein expression of cAMP, PKA, CREB, and cFOS and upregulated the Bcl-2 gene, suggesting that the nephroprotective action of MESFL is due to the suppression of the cAMP/PKA/CREB/cFOS signaling pathway. In addition, MESFL increases SOD and CAT activities and GSH levels, reduces MDA levels, and reduces renal functional indices (ALP, urea, uric acid, and creatinine).
Conclusion: Therefore, our results indicate that MESFL alleviates the development of diabetic nephropathy via suppression of the cAMP/PKA/CREB/cFOS pathways.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Ojo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje