Pharmacological Evidence of Eugenia Brasiliensis Leaves in a Reserpine-Induced Fibromyalgia Model: Antinociceptive, Emotional, Anti-Inflammatory, and Neurotrophic Effects.

Autor: Kraus SI; Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil.; Post-Graduate Program in Neurosciences, Center for Biological Sciences, Federal University of Santa Catarina, University Campus, Trindade, Florian?polis, SC, 88040-900, Brazil., Ferreira JB; Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil.; Post-Graduate Program in Neurosciences, Center for Biological Sciences, Federal University of Santa Catarina, University Campus, Trindade, Florian?polis, SC, 88040-900, Brazil., França AP; Signal Transduction Laboratory, Department of Biochemistry, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil.; Graduate Program in Medical Sciences, Health Sciences Center (CCS), University Hospital (HU), Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-970, Brazil., Wippel VA; Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil.; Post-Graduate Program in Neurosciences, Center for Biological Sciences, Federal University of Santa Catarina, University Campus, Trindade, Florian?polis, SC, 88040-900, Brazil., Leal RB; Signal Transduction Laboratory, Department of Biochemistry, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil.; Post-Graduate Program in Neurosciences, Center for Biological Sciences, Federal University of Santa Catarina, University Campus, Trindade, Florian?polis, SC, 88040-900, Brazil., Oppermann R; Department of Pharmaceutical Sciences, Campus III, Regional University of Blumenau- Rua S?o Paulo 2171, Itoupava Seca, 89030-003, Brazil., Alberton MD; Department of Pharmaceutical Sciences, Campus III, Regional University of Blumenau- Rua S?o Paulo 2171, Itoupava Seca, 89030-003, Brazil., da Silva MD; Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, Florian?polis, SC, 88040-900, Brazil. morgana.silva@ufsc.br.; Post-Graduate Program in Neurosciences, Center for Biological Sciences, Federal University of Santa Catarina, University Campus, Trindade, Florian?polis, SC, 88040-900, Brazil. morgana.silva@ufsc.br.
Jazyk: angličtina
Zdroj: Molecular neurobiology [Mol Neurobiol] 2024 Nov; Vol. 61 (11), pp. 9402-9415. Date of Electronic Publication: 2024 Apr 19.
DOI: 10.1007/s12035-024-04167-8
Abstrakt: Fibromyalgia (FM) is a painful chronic condition that significantly impacts the quality of life, posing challenges for clinical management. Given the difficulty of understanding the pathophysiology and finding new therapeutics, this study explored the effects of a medicinal plant, E. brasiliensis, in an FM model induced by reserpine in Swiss mice. Animals were treated with saline 0.9% (vehicle), duloxetine 10 mg/kg (positive control), or hydroalcoholic extract of E. brasiliensis leaves 300 mg/kg (HEEb). Nociceptive parameters, as well as locomotion, motor coordination, strength, anxiety, and depressive-like behaviors, were evaluated for 10 days. After that, the brain and blood were collected for further analysis of cytokines (interleukin 1? and interleukin 6), brain-derived neurotrophic factor (BDNF), and the immunocontents of total and phosphorylated Tropomyosin receptor kinase B (TrkB). The results demonstrated that the acute and prolonged treatment with HEEb was able to reduce both mechanical and thermal nociception. It was also possible to observe an increase in the strength, without changing locomotion and motor coordination parameters. Interestingly, treatment with HEEb reduces anxious and depressive-like behaviors. Finally, we observed a reduction in inflammatory cytokines in the hippocampus of animals treated with HEEb, while an increase in BDNF was observed in the prefrontal cortex (PFC). However, no alterations related to total and phosphorylated TrkB receptor expression were found. Our study demonstrated the antinociceptive and emotional effects of HEEb in mice, possibly acting on neuroinflammatory and neurotrophic mechanisms. These data provide initial evidence about the E. brasiliensis potential for treating chronic pain.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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