Impact of carbimazole combined with vitamin E on testicular injury induced by experimental hyperthyroidism in adult albino rats: oxidative/inflammatory/apoptotic pathways.

Autor:	Hussein RS; Department of Dermatology, College of Medicine Prince Sattam Bin Abdulaziz University, Al-Kharj 16242, Saudi Arabia.; Department of Dermatology, Andrology and STDs, Assiut Police Hospital, Assiut 71525, Egypt., Eyada MM; Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt., Mostafa RM; Department of Andrology and Sexology, Faculty of Medicine, Suez Canal University, Ismailia 41511, Egypt., Elaidy SM; Department of Clinical Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia 41511, Egypt., Elsayed SH; Department of Rehabilitation Sciences, Faculty of Health and Rehabilitation Sciences, Princess Nourah bint Abdulrahman University, Riyadh 12271, Saudi Arabia., Saad HM; Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
Jazyk:	angličtina
Zdroj:	Asian journal of andrology [Asian J Androl] 2024 Jul 01; Vol. 26 (4), pp. 396-401. Date of Electronic Publication: 2024 Apr 19.
DOI:	10.4103/aja202365
Abstrakt:	Thyroid hormones play essential roles in spermatogenesis, but their effects on infertile males remain poorly understood. This study aimed to evaluate the impact of combining carbimazole (CBZ) with vitamin E (VE) on testicular injury induced by experimental hyperthyroidism in adult albino rats, focusing on oxidative, inflammatory, and apoptotic pathways. In this experimental study, 64 adult male albino Wistar rats were divided into eight groups: Group I (control-untreated), Group II (CBZ-control), Group III (VE-control), Group IV (CBZ + VE-control), Group V (levothyroxine-induced testicular injury), Group VI (levothyroxine + CBZ-treated), Group VII (levothyroxine + VE-treated), and Group VIII (levothyroxine + CBZ + VE-treated). The study was conducted in the Faculty of Medicine, Suez Canal University (Ismailia, Egypt). After cervical decapitation, both testes and epididymis were examined histopathologically and immunohistochemically. Significant differences were observed among groups concerning malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT; all P < 0.001). Polymerase chain reaction analysis showed significant differences in tumor necrosis factor-α (TNF- α ), interleukin-10 (IL-10), Bcl-2-associated X protein (BAX), B-cell lymphoma 2 protein (Bcl2), p53, Caspase-3, Caspase-8, Caspase-9, and nuclear factor-kappa B (NF- κ B) mRNA levels (all P < 0.001). Hyperthyroid group treated with CBZ alone (Group VI) exhibited testicular side effects, affecting seminiferous tubules and spermatogenesis. However, the Group VIII showed improved spermatogenesis and a decrease in testicular side effects. The addition of VE to the treatment of hyperthyroid rats with CBZ reduced testicular side effects and seminiferous tubular affection when potentially improving spermatogenesis. Further research is needed to elucidate the underlying mechanisms fully. (Copyright © 2024 Copyright: ©The Author(s)(2024).)
Databáze:	MEDLINE
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