Exploring untapped bacterial communities and potential polypropylene-degrading enzymes from mangrove sediment through metagenomics analysis.

Autor:	Pawano O; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.; Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology, Bangkok, Thailand., Jenpuntarat N; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.; Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology, Bangkok, Thailand., Streit WR; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany., Pérez-García P; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany.; Molecular Microbiology, Institute of General Microbiology, Kiel University, Kiel, Germany., Pongtharangkul T; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand., Phinyocheep P; Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand., Thayanukul P; Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.; Faculty of Science, Center of Excellence for Vectors and Vector-Borne Diseases, Mahidol University at Salaya, Nakhon Pathom, Thailand., Euanorasetr J; Laboratory of Biotechnological Research for Energy and Bioactive Compound (BREBC), Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok, Thailand., Intra B; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.; Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology, Bangkok, Thailand.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Apr 04; Vol. 15, pp. 1347119. Date of Electronic Publication: 2024 Apr 04 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1347119
Abstrakt:	The versatility of plastic has resulted in huge amounts being consumed annually. Mismanagement of post-consumption plastic material has led to plastic waste pollution. Biodegradation of plastic by microorganisms has emerged as a potential solution to this problem. Therefore, this study aimed to investigate the microbial communities involved in the biodegradation of polypropylene (PP). Mangrove soil was enriched with virgin PP sheets or chemically pretreated PP comparing between 2 and 4 months enrichment to promote the growth of bacteria involved in PP biodegradation. The diversity of the resulting microbial communities was accessed through 16S metagenomic sequencing. The results indicated that Xanthomonadaceae, unclassified Gaiellales, and Nocardioidaceae were promoted during the enrichment. Additionally, shotgun metagenomics was used to investigate enzymes involved in plastic biodegradation. The results revealed the presence of various putative plastic-degrading enzymes in the mangrove soil, including alcohol dehydrogenase, aldehyde dehydrogenase, and alkane hydroxylase. The degradation of PP plastic was determined using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM), and Water Contact Angle measurements. The FTIR spectra showed a reduced peak intensity of enriched and pretreated PP compared to the control. SEM images revealed the presence of bacterial biofilms as well as cracks on the PP surface. Corresponding to the FTIR and SEM analysis, the water contact angle measurement indicated a decrease in the hydrophobicity of PP and pretreated PP surface during the enrichment. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Pawano, Jenpuntarat, Streit, Pérez-García, Pongtharangkul, Phinyocheep, Thayanukul, Euanorasetr and Intra.)
Databáze:	MEDLINE
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