Insights into measles virus: Serological surveillance and molecular characterization.

Autor:	Khalid Z; Department of Microbiology, The University of Haripur, Haripur, KP 22610, Pakistan. Electronic address: zainabkhalid675@gmail.com., Muhammad J; Department of Microbiology, The University of Haripur, Haripur, KP 22610, Pakistan. Electronic address: javed.muhammad@uoh.edu.pk., Ali H; Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 200240, China. Electronic address: hinaali.ibs@suit.edu.pk., Rana MS; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: ranavirologist@gmail.com., Usman M; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: usmanmlt@yahoo.com., Alam MM; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: alammu@who.int., Ullah R; Department of Pharmacognosy, College of Pharmacy, King Saud University Riyadh, Saudi Arabia. Electronic address: rullah@ksu.edu.sa., Ullah A; Department of Microbiology, The University of Haripur, Haripur, KP 22610, Pakistan. Electronic address: arif.ullah@uoh.edu.pk., Umair M; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: massab.umair@yahoo.com., Ahmad A; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: ashfaq.2422@gmail.com., Salman M; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: Salman14m@gmail.com., Ikram A; National Institute of Health, Islamabad 45500, Pakistan. Electronic address: maahin1@yahoo.com., Khan A; Department of Public Health and Nutrition, The University of Haripur, Haripur 22610, KP, Pakistan; The University of Kentucky, KY 40506, United States. Electronic address: dramjadkhan77@gmail.com., Bari A; Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University, Riyadh, Saudi Arabia. Electronic address: abari@ksu.edu.sa.
Jazyk:	angličtina
Zdroj:	Journal of infection and public health [J Infect Public Health] 2024 Jun; Vol. 17 (6), pp. 994-1000. Date of Electronic Publication: 2024 Mar 29.
DOI:	10.1016/j.jiph.2024.03.028
Abstrakt:	Background: Measles has been a significant public health concern in Pakistan, especially in the Khyber Pakhtunkhwa (KPK) province, where sporadic and silent epidemics continue to challenge existing control measures. This study aimed to estimate the prevalence and investigate the molecular epidemiology of the measles virus (MeV) in KPK and explore the vaccination status among the suspected individuals. Methods: A cross-sectional study was conducted between February and October 2021. A total of 336 suspected measles cases from the study population were analyzed for IgM antibodies using Enzyme-Linked Immunosorbent Assay (ELISA). Throat swabs were randomly collected from a subset of positive cases for molecular analysis. Phylogenetic analysis of MeV isolates was performed using the neighbor-joining method. The vaccination status of individuals was also recorded. Results: Among the suspected participants, 61.0% (205/336) were ELISA positive for IgM antibodies, with a higher prevalence in males (64.17%) compared to females (57.04%). The majority of cases (36.0%) were observed in infants and toddlers, consistent with previous reports. The majority of IgM-positive cases (71.7%) had not received any dose of measles vaccine, highlighting gaps in vaccine coverage and the need for improved immunization programs. Genetic analysis revealed that all MeV isolates belonged to the B3 genotype, with minor genetic variations from previously reported variants in the region. Conclusion: This study provides valuable insights into the genetic epidemiology of the MeV in KPK, Pakistan. The high incidence of measles infection among unvaccinated individuals highlights the urgency of raising awareness about vaccine importance and strengthening routine immunization programs. Competing Interests: Declaration of Competing Interest All authors have read the manuscript and declare no conflict of interest. (Copyright © 2024. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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