Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance.

Autor:	Xiang X; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Wan ZY; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Zhang S; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Feng QN; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Li SW; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Yin GM; Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China., Zhi JY; Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China., Liang X; Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China., Ma T; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Li S; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Zhang Y; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.; Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Sep 03; Vol. 36 (9), pp. 3419-3434.
DOI:	10.1093/plcell/koae109
Abstrakt:	Protein S-acylation catalyzed by protein S-acyl transferases (PATs) is a reversible lipid modification regulating protein targeting, stability, and interaction profiles. PATs are encoded by large gene families in plants, and many proteins including receptor-like cytoplasmic kinases (RLCKs) and receptor-like kinases (RLKs) are subject to S-acylation. However, few PATs have been assigned substrates, and few S-acylated proteins have known upstream enzymes. We report that Arabidopsis (Arabidopsis thaliana) class A PATs redundantly mediate pollen tube guidance and participate in the S-acylation of POLLEN RECEPTOR KINASE1 (PRK1) and LOST IN POLLEN TUBE GUIDANCE1 (LIP1), a critical RLK or RLCK for pollen tube guidance, respectively. PAT1, PAT2, PAT3, PAT4, and PAT8, collectively named PENTAPAT for simplicity, are enriched in pollen and show similar subcellular distribution. Functional loss of PENTAPAT reduces seed set due to male gametophytic defects. Specifically, pentapat pollen tubes are compromised in directional growth. We determine that PRK1 and LIP1 interact with PENTAPAT, and their S-acylation is reduced in pentapat pollen. The plasma membrane (PM) association of LIP1 is reduced in pentapat pollen, whereas point mutations reducing PRK1 S-acylation affect its affinity with its interacting proteins. Our results suggest a key role of S-acylation in pollen tube guidance through modulating PM receptor complexes. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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