Sensitive detection of HSP70 using a current-amplified biosensor based on antibody-loaded PS-AuNPs@Cys/Au modified ITO chip.

Autor: Liu R; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China., Liu Y; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China., Li C; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China., Agyapong DAY; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China.; Biomedical Engineering Program, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Feng J; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China., Tang L; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China., Zeng H; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, PR China. zenghj@uestc.edu.cn.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Apr 18; Vol. 191 (5), pp. 272. Date of Electronic Publication: 2024 Apr 18.
DOI: 10.1007/s00604-024-06333-0
Abstrakt: A biosensing electrochemical platform for heat shock protein 70 (HSP70) has been developed by integrating a three-electrode indium tin oxide (ITO) on a chip. The platform includes modifications to the reference electrode and working electrode for the detection of HSP70. The new platform is constructed by assembly of HSP70 antibody on PS-AuNPs@Cys/Au indium tin oxide (ITO) electrode to create a high HSP70 sensitive surface. The PS-AuNPs@Cys/Au indium tin oxide (ITO) electrode is obtained by immersing the ITO electrode into the PS-AuNPs@Cys solution and performing constant potential deposition at -1.4 V (Ag/AgCl). The PS-AuNPs@Cys/Au film deposited on ITO glass provides a desirable substrate for the immobilization of the HSP70 antibody and improves the loading of antibody between PS-AuNPs@Cys/Au and the electrode resulting in a significant amplification. Under optimal conditions, the fabricated sensor demonstrates a linear range extending from 0.1 ng mL - 1 to 1000 ng mL - 1 , with an impressive detection limit of 25.7 pg mL - 1 (S/N = 3). The developed immunoassay method successfully detected the HSP70 content in normal human blood samples and outperformed the ELISA method commonly used for clinical sample analysis.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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