DArTseq-based SNP markers reveal high genetic diversity among early generation fall armyworm tolerant maize inbred lines.

Autor: Adu GB; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Awuku FJ; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Garcia-Oliveira AL; International Maize and Wheat Improvement Center (CIMMYT), Nairobi, Kenya.; Department of Molecular Biology, College of Biotechnology, CCS Haryana Agricultural University, Hisar, Haryana, India., Amegbor IK; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana.; Faculty of Natural and Agricultural Sciences, Department of Plant Breeding, University of the Free State, Bloemfontein, South Africa., Nelimor C; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Nboyine J; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Karikari B; Department of Agricultural Biotechnology, Faculty of Agriculture, Food and Consumer Sciences, University for Development Studies, Tamale, Ghana., Atosona B; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Manigben KA; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana., Aboyadana PA; CSIR-Savanna Agricultural Research Institute, Nyankpala, Ghana.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 17; Vol. 19 (4), pp. e0294863. Date of Electronic Publication: 2024 Apr 17 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0294863
Abstrakt: Diversity analysis using molecular markers serves as a powerful tool in unravelling the intricacies of inclusivity within various populations and is an initial step in the assessment of populations and the development of inbred lines for host plant resistance in maize. This study was conducted to assess the genetic diversity and population structure of 242 newly developed S3 inbred lines using 3,305 single nucleotide polymorphism (SNP) markers and to also assess the level of homozygosity achieved in each of the inbred lines. A total of 1,184 SNP markers were found highly informative, with a mean polymorphic information content (PIC) of 0.23. Gene diversity was high among the inbred lines, ranging from 0.04 to 0.50, with an average of 0.27. The residual heterozygosity of the 242 S3 inbred lines averaged 8.8%, indicating moderately low heterozygosity levels among the inbred lines. Eighty-four percent of the 58,322 pairwise kinship coefficients among the inbred lines were near zero (0.00-0.05), with only 0.3% of them above 0.50. These results revealed that many of the inbred lines were distantly related, but none were redundant, suggesting each inbred line had a unique genetic makeup with great potential to provide novel alleles for maize improvement. The admixture-based structure analysis, principal coordinate analysis, and neighbour-joining clustering were concordant in dividing the 242 inbred lines into three subgroups based on the pedigree and selection history of the inbred lines. These findings could guide the effective use of the newly developed inbred lines and their evaluation in quantitative genetics and molecular studies to identify candidate lines for breeding locally adapted fall armyworm tolerant varieties in Ghana and other countries in West and Central Africa.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Adu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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