| Autor: |
Shabbir MZ; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA.; Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan., Yu H; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA.; Tianjin Ringpu Bio-Technology Co, Ltd., Tianjin, People's Republic of China., Lighty ME; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA., Dunn PA; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA., Wallner-Pendleton EA; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA., Lu H; Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA, USA. |
| Abstrakt: |
Avian reovirus (ARV) has been continuously affecting the poultry industry in Pennsylvania (PA) in recent years. This report provides our diagnostic investigation on monitoring ARV field variants from broiler chickens in Pennsylvania. Genomic characterization findings of 72 ARV field isolates obtained from broiler cases during the last 6 years indicated that six distinct cluster variant strains (genotype I-VI), which were genetically diverse and distant from the vaccine and vaccine-related field strains, continuously circulated in PA poultry. Most of the variants clustered within genotype V (24/72, 33.3%), followed by genotype II (16/72, 22.2%), genotype IV (13/72, 18.1%), genotype III (13/72, 18.1%), genotype VI (05/72, 6.94%), and genotype I (1/72, 1.38%). The amino acid identity between 72 field variants and the vaccine strains (1133, 1733, 2408, 2177) varied from 45.3% to 99.7%, while the difference in amino acid counts ranged from 1-164. Among the field variants, the amino acid identity and count difference ranged from 43.3% to 100% and 0 to 170, respectively. Variants within genotype V had maximum amino acid identity (94.7-100%), whereas none of the variants within genotypes II and VI were alike. These findings indicate the continuing occurrence of multiple ARV genotypes in the environment. |
