Metabolic and ultrastructural renal changes in adult Wistar rats fed by a cafeteria diet.

Autor:	Santos PFD; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil., Souza DB; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil., Torres EJL; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Microbiology, Immunology and Parasitology - Rio de Janeiro (RJ) - Brazil., Costa WS; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil., Sampaio FJB; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil., Gregorio BM; Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2024 Apr 15; Vol. 39, pp. e392224. Date of Electronic Publication: 2024 Apr 15 (Print Publication: 2024).
DOI:	10.1590/acb392224
Abstrakt:	Purpose: To evaluate, by quantitative and qualitative methods, the glomerular ultrastructure in Wistar rats fed a cafeteria diet. Methods: Male Wistar rats were divided into two groups at 21 days of age: control (C, n = 10) and cafeteria diet (CAF, n = 8). The animals were followed up until 5 months of age, followed by euthanasia. The blood, kidneys, and fat deposits--epididymal, retroperitoneal, and subcutaneous--were extracted and analyzed. Data were analyzed by Student's t test, and p < 0.05 was considered statistically significant. Results: The cafeteria diet promoted glucose intolerance, hyperglycemia (p < 0.0001), and deposition of retroperitoneal fat (p < 0.005). Scanning electron microscopy revealed that the length of the foot process was similar in both groups. The quantitative analyses by transmission electron microscopy revealed that the cafeteria diet reduced the thickness of the glomerular basement membrane (p < 0.05). Conclusions: The intake of lipids and simple carbohydrates were found to be associated with alteration in the glomerular ultrastructure. However, more studies are needed to evaluate not only the effects of high-protein and high-fat diets on components of the glomerular filtration barrier, but also renal physiology.
Databáze:	MEDLINE
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