Genomic analysis of KEL*03 and KEL*04 alleles among Thai blood donors.
| Autor: | Nathalang O; Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand., Rassuree P; Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand., Intharanut K; Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand., Chaibangyang W; Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand., Nogués N; Laboratori d'Immunohematologia Banc de Sang i Teixits, Barcelona, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | African journal of laboratory medicine [Afr J Lab Med] 2024 Mar 19; Vol. 13 (1), pp. 2294. Date of Electronic Publication: 2024 Mar 19 (Print Publication: 2024). |
| DOI: | 10.4102/ajlm.v13i1.2294 |
| Abstrakt: | Background: The Kell blood group system is clinically important in transfusion medicine, particularly in patients with antibodies specific to Kell antigens. To date, genetic variations of the Kell metallo-endopeptidase ( KEL ) gene among Thai populations remain unknown. Objective: This study aimed to determine the frequencies of KEL*03 and KEL*04 alleles among Thai blood donors using an in-house polymerase chain reaction-sequence-specific primer (PCR-SSP) method. Methods: Blood samples obtained from 805 unrelated central Thai blood donors at a blood bank in Pathumthani, Thailand, from March 2023 to June 2023, were typed for Kp a and Kp b antigens using the column agglutination test, and the results for 400 samples were confirmed using DNA sequencing. A PCR-SSP method was developed to detect the KEL*03 and KEL*04 alleles, and genotyping results were validated using known DNA controls. DNA samples obtained from Thai donors in central ( n = 2529), northern ( n = 300), and southern ( n = 427) Thailand were also genotyped using PCR-SSP for comparison. Results: All 805 (100%) donors had the Kp(a-b+) phenotype. The PCR-SSP genotyping results agreed with the column agglutination test and DNA sequencing. All 3256 Thai blood donors had the homozygous KEL*04/KEL*04 genotype. Frequencies of the KEL*03 and KEL*04 alleles among Thai donors differed significantly from those of Japanese, Native American, South African, Brazilian, Swiss, and German populations. Conclusion: This study found a 100% KEL*04 allele frequency in three Thai populations. These data could provide information on KEL*03 and KEL*04 allele frequencies to estimate the risk of alloimmunisation in Thai populations. What This Study Adds: This study demonstrates that in-house PCR-SSP can be used to determine KEL*03 and KEL*04 alleles to predict Kp a and Kp b antigens. Even though only homozygous KEL*04/KEL*04 genotypes were found among Thai donor populations, the established PCR-SSP method may be useful for estimating the risk of alloimmunisation in other populations. Competing Interests: The authors declare that they have no financial or personal relationships that may have appropriately influenced them in writing this article. (© 2024. The Authors.) |
| Databáze: | MEDLINE |
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