Complement C5a Receptor Signaling Alters Stress Responsiveness and Modulates Microglia Following Chronic Stress Exposure.

Autor:	Chen HC; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia.; Metabolic Research Laboratories, Wellcome Trust MRC Institute of Metabolic Science, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom., Spiers JG; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.; Clear Vision Research, Eccles Institute of Neuroscience, John Curtin School of Medical Research, College of Health and Medicine, the Australian National University, Acton, Australian Capital Territory, Australia.; School of Medicine and Psychology, College of Health and Medicine, the Australian National University, Australian Capital Territory, Australia., Lerskiatiphanich T; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia., Parker SE; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia., Lavidis NA; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia., Fung JN; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia., Woodruff TM; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia.; Queensland Brain Institute, the University of Queensland, St Lucia, Brisbane, Queensland, Australia., Lee JD; School of Biomedical Sciences, the University of Queensland, St. Lucia, Brisbane, Queensland, Australia.
Jazyk:	angličtina
Zdroj:	Biological psychiatry global open science [Biol Psychiatry Glob Open Sci] 2024 Mar 07; Vol. 4 (3), pp. 100306. Date of Electronic Publication: 2024 Mar 07 (Print Publication: 2024).
DOI:	10.1016/j.bpsgos.2024.100306
Abstrakt:	Background: Accumulating evidence underscores the pivotal role of heightened inflammation in the pathophysiology of stress-related diseases, but the underlying mechanisms remain elusive. The complement system, a key effector of the innate immune system, produces the C5-cleaved activation product C5a upon activation, initiating inflammatory responses through the canonical C5a receptor 1 (C5aR1). While C5aR1 is expressed in stress-responsive brain regions, its role in stress responsiveness remains unknown. Methods: To investigate C5a-C5aR1 signaling in stress responses, mice underwent acute and chronic stress paradigms. Circulating C5a levels and messenger RNA expression of C5aR1 in the hippocampus and adrenal gland were measured. C5aR1-deficient mice were used to elucidate the effects of disrupted C5a-C5aR1 signaling across behavioral, hormonal, metabolic, and inflammation parameters. Results: Chronic restraint stress elevated circulating C5a levels while reducing C5aR1 messenger RNA expression in the hippocampus and adrenal gland. Notably, the absence of C5aR1 signaling enhanced adrenal sensitivity to adrenocorticotropic hormone, concurrently reducing pituitary adrenocorticotropic hormone production and enhancing the response to acute stress. C5aR1-deficient mice exhibited attenuated reductions in locomotor activity and body weight under chronic stress. Additionally, these mice displayed increased glucocorticoid receptor sensitivity and disrupted glucose and insulin homeostasis. Chronic stress induced an increase in C5aR1-expressing microglia in the hippocampus, a response mitigated in C5aR1-deficient mice. Conclusions: C5a-C5aR1 signaling emerges as a key metabolic regulator during stress, suggesting that complement activation and dysfunctional C5aR1 signaling may contribute to neuroinflammatory phenotypes in stress-related disorders. The results advocate for further exploration of complement C5aR1 as a potential therapeutic target for stress-related conditions. (© 2024 The Authors.)
Databáze:	MEDLINE
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