Integration of a biocompatible metal-phenolic network and fluorescence microspheres as labels for sensitive and stable detection of carbendazim with a lateral flow immunoassay.

Autor: Chen Z; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China., Tang Y; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China., Guo P; Jiangxi General Institute of Testing and Certification, Nanchang 330029, China., Zhang W; Jiangxi General Institute of Testing and Certification, Nanchang 330029, China., Peng J; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China., Xiong Y; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China., Ma B; Jiangxi General Institute of Testing and Certification, Nanchang 330029, China. Electronic address: 569211446@qq.com., Lai W; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China. Electronic address: talktolaiwh@163.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Aug 30; Vol. 450, pp. 139260. Date of Electronic Publication: 2024 Apr 04.
DOI: 10.1016/j.foodchem.2024.139260
Abstrakt: High fluorescence intensity microspheres such as aggregation-induced emission fluorescence microspheres (AIEFM) have improved the sensitivity of lateral flow immunoassay (LFIA). The preparation of immune probes in LFIA usually adopts the chemical coupling strategy with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for antibody coupling, which has the problems of low coupling efficiency, tedious coupling process, and poor repeatability. A biocompatible metal-phenolic network (MPN), which contains large amounts of phenols and galloyl groups, could easily, quickly, and stably couple with antibodies. Herein, we proposed a strategy based on MPN modification on ultrabright AIEFM surface as a novel label for the rapid detection of carbendazim. The limit of detection of AIEFM@MPN-LFIA was 0.019 ng/mL, which was 4.9 times lower than that of AIEFM-LFIA. In spiked samples, the average recoveries of AIEFM@MPN-LFIA ranged from 80% to 118% (coefficient of variation <13.45%). Therefore, AIEFM@MPN was a promising signal label that could improve the detection performance of LFIA.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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