Global changes in gene expression during compatible and incompatible interactions of faba bean (Vicia faba L.) during Orobanche foetida parasitism.

Autor: Boukteb A; Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia.; Field Crop Laboratory, National Institute of Agricultural Research of Tunisia, Carthage University, Tunis, Tunisia., Sato K; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Gan P; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Kharrat M; Field Crop Laboratory, National Institute of Agricultural Research of Tunisia, Carthage University, Tunis, Tunisia., Sakouhi H; Field Crop Laboratory, National Institute of Agricultural Research of Tunisia, Carthage University, Tunis, Tunisia., Shibata A; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Shirasu K; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Ichihashi Y; RIKEN BioResource Research Center, Tsukuba, Japan., Bouhadida M; Field Crop Laboratory, National Institute of Agricultural Research of Tunisia, Carthage University, Tunis, Tunisia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 16; Vol. 19 (4), pp. e0301981. Date of Electronic Publication: 2024 Apr 16 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0301981
Abstrakt: Orobanche foetida Poiret is the main constraint facing faba bean crop in Tunisia. Indeed, in heavily infested fields with this parasitic plant, yield losses may reach 90%, and the recent estimation of the infested area is around 80,000 ha. Identifying genes involved in the Vicia faba/O. foetida interaction is crucial for the development of effective faba bean breeding programs. However, there is currently no available information on the transcriptome of faba bean responding to O. foetida parasitism. In this study, we employed RNA sequencing to explore the global gene expression changes associated with compatible and incompatible V. faba/O. foetida interactions. In this perspective, two faba bean varieties (susceptible and resistant) were examined at the root level across three stages of O. foetida development (Before Germination (BG), After Germination (AG) and Tubercule Stage (TS)). Our analyses presented an exploration of the transcriptomic profile, including comprehensive assessments of differential gene expression and Gene Ontology (GO) enrichment analyses. Specifically, we investigated key pathways revealing the complexity of molecular responses to O. foetida attack. In this study, we detected differential gene expression of pathways associated with secondary metabolites: flavonoids, auxin, thiamine, and jasmonic acid. To enhance our understanding of the global changes in V. faba response to O. foetida, we specifically examined WRKY genes known to play a role in plant host-parasitic plant interactions. Furthermore, considering the pivotal role of parasitic plant seed germination in this interaction, we investigated genes involved in the orobanchol biosynthesis pathway. Interestingly, we detected the gene expression of VuCYP722C homolog, coding for a key enzyme involved in orobanchol biosynthesis, exclusively in the susceptible host. Clearly, this study enriches our understanding of the V. faba/O. foetida interaction, shedding light on the main differences between susceptible and resistant faba bean varieties during O. foetida infestation at the gene expression level.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Boukteb et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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