Adhesion and biofilm formation by two clinical isolates of Trichosporon Cutaneum in various environmental conditions.

Autor: Piecuch A; Department of Mycology and Genetics, University of Wrocław, Przybyszewskiego Str 63/77, Wrocław, Poland. agata.piecuch@uwr.edu.pl., Cal M; Department of Mycology and Genetics, University of Wrocław, Przybyszewskiego Str 63/77, Wrocław, Poland., Ogórek R; Department of Mycology and Genetics, University of Wrocław, Przybyszewskiego Str 63/77, Wrocław, Poland.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1793-1800. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1007/s42770-024-01321-1
Abstrakt: Trichosporon spp. is an emerging opportunistic pathogen and a common cause of both superficial and invasive infections. Although Trichosporon asahii is the most frequently isolated species, Trichosporon cutaneum is also widely observed, as it is the predominant agent in cases of white Piedra and onychomycosis. Trichosporon spp. is a known to produce biofilms, which serve as one of its virulence mechanisms, however, there is limited data available on biofilms formed by T. cutaneum. Thus, the aim of this study was to assess the adhesion and biofilm formation of two clinical isolates of T. cutaneum under various environmental conditions (including temperature, nutrient availability, and carbon source), as well as their tolerance to fluconazole. Adhesion was tested on common abiotic substrates (such as silicone, glass, and stainless steel), revealing that T. cutaneum readily adhered to all surfaces tested. CV staining was applied for the evaluation of the environment influence on biofilm efficiency and it was proved that the nutrient availability has a major impact. Additionaly, fluorescent staining was employed to visualize the morphology of T. cutaneum biofilm and its survival in the presence of fluconazole. Hyphae production was shown to play a role in elevated biofilm production in minimal medium and increased tolerance to fluconazole.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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