A flow cytometry-based assay to measure neutralizing antibodies against SARS-CoV-2 virus.

Autor: Pscheidt VM; Immunotherapy Laboratory - (LAIT) - Department of Basic Health Sciences of Federal University of Health Sciences of Porto Alegre, UFCSPA, Porto Alegre, Brazil., de Souza PO; Immunotherapy Laboratory - (LAIT) - Department of Basic Health Sciences of Federal University of Health Sciences of Porto Alegre, UFCSPA, Porto Alegre, Brazil., Fazolo T; Immunotherapy Laboratory - (LAIT) - Department of Basic Health Sciences of Federal University of Health Sciences of Porto Alegre, UFCSPA, Porto Alegre, Brazil., Modena JLP; Laboratory of Emerging Viruses (LEVE) - Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas - UNICAMP - Campinas, São Paulo, Brazil., Simeoni C; Laboratory of Emerging Viruses (LEVE) - Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas - UNICAMP - Campinas, São Paulo, Brazil., Teixeira D; Laboratory of Emerging Viruses (LEVE) - Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas - UNICAMP - Campinas, São Paulo, Brazil., Silva NB; Laboratory of Emerging Viruses (LEVE) - Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas - UNICAMP - Campinas, São Paulo, Brazil., Dos Santos KB; Laboratory of Emerging Viruses (LEVE) - Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas - UNICAMP - Campinas, São Paulo, Brazil., Júnior LR; Immunotherapy Laboratory - (LAIT) - Department of Basic Health Sciences of Federal University of Health Sciences of Porto Alegre, UFCSPA, Porto Alegre, Brazil., Bonorino C; Immunotherapy Laboratory - (LAIT) - Department of Basic Health Sciences of Federal University of Health Sciences of Porto Alegre, UFCSPA, Porto Alegre, Brazil.; Department of Surgery, University of California at San Diego - UCSD, La Jolla, California, USA.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2024 Jun; Vol. 105 (6), pp. 446-457. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1002/cyto.a.24838
Abstrakt: The COVID-19 pandemic caused by the SARS-CoV-2 virus has highlighted the need for serological assays that can accurately evaluate the neutralizing efficiency of antibodies produced during infection or induced by vaccines. However, conventional assays often require the manipulation of live viruses on a level-three biosafety (BSL3) facility, which presents practical and safety challenges. Here, we present a novel, alternative assay that measures neutralizing antibodies (NAbs) against SARS-CoV-2 in plasma using flow cytometry. This assay is based on antibody binding to the S protein and has demonstrated precision in both intra- and inter-assay measurements at a dilution of 1:50. The cut-off was determined using Receiver Operating Characteristic (ROC) analysis and the value of 36.01% has shown high sensitivity and specificity in distinguishing between pre-pandemic sera, COVID-19 patients, and vaccinated individuals. The efficiency significantly correlates with the gold standard test, PRNT. Our new assay offers a safe and efficient alternative to conventional assays for evaluating NAbs against SARS-CoV-2.
(© 2024 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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