Caffeine quantification in dietary supplements using high-throughput on-line solid phase extraction coupled to Venturi easy ambient sonic-spray ionization mass spectrometry.

Autor: Tega DU; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br., Campos Oliveira LF; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br., Ferreira PC; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br., Soldera BB; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br., Nascimento HDL; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br., Eberlin MN; School of Engineering, Mackenzie Presbyterian University, Rua da Consolação 930, 01302-907, São Paulo, SP, Brazil.; Mackenzie Institute for Research in Graphene and Nanotechnologies (MackGraphe), Rua da Consolação 896, 01302-907, São Paulo, SP, Brazil., Sussulini A; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. sussulini@unicamp.br.; Instituto Nacional de Ciência e Tecnologia de Bioanalítica (INCTBio), Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil.
Jazyk: angličtina
Zdroj: Analytical methods : advancing methods and applications [Anal Methods] 2024 May 03; Vol. 16 (17), pp. 2678-2683. Date of Electronic Publication: 2024 May 03.
DOI: 10.1039/d4ay00333k
Abstrakt: Caffeine is present in a large number of beverages and is an additive used in dietary supplements. Therefore, the concern about its quality and safety for consumers has been increasing and hence requires faster and simpler analytical methods to determine the caffeine amount. The high-throughput analysis is an appropriate solution to pharmaceuticals, bioanalysis, forensic and food laboratory routines. In this sense, Venturi easy ambient sonic-spray ionization mass spectrometry (V-EASI-MS), a specific ambient ionization source, is suitable to enable direct analysis of sample solutions in real time and is appropriate to be coupled to liquid chromatography (LC). The development of an on-line solid phase extraction system coupled to V-EASI-MS optimizes the advantages of LC-MS hyphenation by enhancing the figures of merit of the analytical method according to AOAC guidelines and simultaneously minimizing the runtime analysis to 1.5 min per sample, as well as sample preparation steps and solvent consumption, which is currently a challenge for quantitative applications of ambient ionization MS.
Databáze: MEDLINE